Transcriptional Basis of Ca[sup.2+] Remodeling Reversal Induced by Polyamine Synthesis Inhibition in Colorectal Cancer Cells

The common activation of the c-Myc oncogene in colorectal cancer (CRC) induces the overexpression of ornithine decarboxylase (ODC), the limiting step in polyamine synthesis, which is a process blocked by α-Difluoromethylornithine (DFMO), an ODC suicide inhibitor and potential CRC treatment. We showed previously that intracellular Ca[sup.2+] homeostasis is remodeled in CRC and contributes to cancer hallmarks. We investigated whether polyamine synthesis inhibition induced by DFMO may reverse this remodeling in CRC and, if so, the molecular basis for this phenotypic reversal. To this end we used calcium imaging and transcriptomic analysis in both normal and CRC cells. We found that CRC cells showed enhanced resting Ca[sup.2+] and store-operated Ca[sup.2+] entry (SOCE) but decreased Ca[sup.2+] store content relative to normal cells. Polyamine synthesis inhibition reversed not only this Ca[sup.2+] remodeling but also reversed the changes in the transcription of a dozen genes involved in Ca[sup.2+] transport in CRC cells, including genes modulating Ca[sup.2+] entry into the cells as store-operated channels and TRP channels as well as Ca[sup.2+] extrusion systems in the plasma membrane and mitochondria. These results provide a molecular basis for the role of polyamine synthesis in Ca[sup.2+] remodeling in cancer. Colorectal cancer (CRC) is associated with mutations in APC/Wnt leading to c-myc activation and the overexpression of ODC1, the limiting step in polyamine synthesis. CRC cells also display a remodeling of intracellular Ca[sup.2+] homeostasis that contributes to cancer hallmarks. As polyamines may modulate Ca[sup.2+] homeostasis during epithelial tissue repair, we investigated whether polyamine synthesis inhibition may reverse Ca[sup.2+] remodeling in CRC cells and, if so, the molecular basis for this reversal. To this end, we used calcium imaging and transcriptomic analysis in normal and CRC cells treated with DFMO, an ODC1 suicide inhibitor. We found that polyamine synthesis inhibition partially reversed changes in Ca[sup.2+] homeostasis associated with CRC, including a decrease in resting Ca[sup.2+] and SOCE along with an increased Ca[sup.2+] store content. We also found that polyamine synthesis inhibition reversed transcriptomic changes in CRC cells without affecting normal cells. Specifically, DFMO treatment enhanced the transcription of SOCE modulators CRACR2A; ORMDL3; and SEPTINS 6, 7, 8, 9, and 11, whereas it decreased SPCA2, involved in store-ind