Significance of Breast Cancer Stem Cell Marker and Tumor Suppressor miRNAs in Breast Carcinoma: A Step Toward Precision Medicine
Breast cancer is a complex disease with heterogeneity and several studies have been conducted to explore differentially expressed miRNA in carcinogenesis. Tumor suppressor miRNAs (miR 200a, miR 200b, miR205 and miR 145) are involved in various signalling pathways and promote carcinogenesis and Cance...
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| Veröffentlicht in: | Indian journal of clinical biochemistry 2022-05, Vol.34 (S1), p.S101 |
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| creator | Dwivedi, Shailendra Pareek, Puneet Vishnoi, Jeevan Ram Sharma, Praveen Misra, Sanjeev |
| description | Breast cancer is a complex disease with heterogeneity and several studies have been conducted to explore differentially expressed miRNA in carcinogenesis. Tumor suppressor miRNAs (miR 200a, miR 200b, miR205 and miR 145) are involved in various signalling pathways and promote carcinogenesis and Cancer stem cells (CSCs) noticed as the driving force of tumorigenesis and metastases. Thus, my objective was to explore relationship of expressed miRNAs and Cancer Stem Cells in breast cancer patients before and after chemotherapy. 39 Breast Cancer samples were recruited after pathological approval and ethical clarification. miRNA was quantified on real-time PCR by using exiqon cDNA and Sybr green kit. CSCs (CD44+/CD24-) were characterized by using CD44 and CD24 antibodies on BD flow cytometer. Breast Cancer Stem Cell marker CD44+/CD24- were significantly reduced after three cycle of chemotherapy (Average% & Mean counts: 7.60% & 590 Vs 3.22% & 291). However, the highest frequency of cells with expression of CD44-/CD24+ were observed and remain almost unchanged after 3 cycle of chemotherapy (Average % & Mean counts: 33.68% & 23,953 Vs 32.63% & 21,648). The Breast cancer patients showed significant (p |
| format | Article |
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Tumor suppressor miRNAs (miR 200a, miR 200b, miR205 and miR 145) are involved in various signalling pathways and promote carcinogenesis and Cancer stem cells (CSCs) noticed as the driving force of tumorigenesis and metastases. Thus, my objective was to explore relationship of expressed miRNAs and Cancer Stem Cells in breast cancer patients before and after chemotherapy. 39 Breast Cancer samples were recruited after pathological approval and ethical clarification. miRNA was quantified on real-time PCR by using exiqon cDNA and Sybr green kit. CSCs (CD44+/CD24-) were characterized by using CD44 and CD24 antibodies on BD flow cytometer. Breast Cancer Stem Cell marker CD44+/CD24- were significantly reduced after three cycle of chemotherapy (Average% & Mean counts: 7.60% & 590 Vs 3.22% & 291). However, the highest frequency of cells with expression of CD44-/CD24+ were observed and remain almost unchanged after 3 cycle of chemotherapy (Average % & Mean counts: 33.68% & 23,953 Vs 32.63% & 21,648). The Breast cancer patients showed significant (p<0.5) down-regulated expression of miR 21 (Mean Cq 27.95 [+ or -] 1.63 Vs 26.51 [+ or -] 1.00) after 3 cycle of standard chemotherapy out of four tumor suppressor mir-200a, mir-200b, mir-205 and mir-145). This study had shown the all tumor suppressor miRNAs 205 showed higher expression with decrease in mean count of CSCs (CD44+/CD24-) in patients positively responding therapy. 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However, the highest frequency of cells with expression of CD44-/CD24+ were observed and remain almost unchanged after 3 cycle of chemotherapy (Average % & Mean counts: 33.68% & 23,953 Vs 32.63% & 21,648). The Breast cancer patients showed significant (p<0.5) down-regulated expression of miR 21 (Mean Cq 27.95 [+ or -] 1.63 Vs 26.51 [+ or -] 1.00) after 3 cycle of standard chemotherapy out of four tumor suppressor mir-200a, mir-200b, mir-205 and mir-145). This study had shown the all tumor suppressor miRNAs 205 showed higher expression with decrease in mean count of CSCs (CD44+/CD24-) in patients positively responding therapy. 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Tumor suppressor miRNAs (miR 200a, miR 200b, miR205 and miR 145) are involved in various signalling pathways and promote carcinogenesis and Cancer stem cells (CSCs) noticed as the driving force of tumorigenesis and metastases. Thus, my objective was to explore relationship of expressed miRNAs and Cancer Stem Cells in breast cancer patients before and after chemotherapy. 39 Breast Cancer samples were recruited after pathological approval and ethical clarification. miRNA was quantified on real-time PCR by using exiqon cDNA and Sybr green kit. CSCs (CD44+/CD24-) were characterized by using CD44 and CD24 antibodies on BD flow cytometer. Breast Cancer Stem Cell marker CD44+/CD24- were significantly reduced after three cycle of chemotherapy (Average% & Mean counts: 7.60% & 590 Vs 3.22% & 291). However, the highest frequency of cells with expression of CD44-/CD24+ were observed and remain almost unchanged after 3 cycle of chemotherapy (Average % & Mean counts: 33.68% & 23,953 Vs 32.63% & 21,648). The Breast cancer patients showed significant (p<0.5) down-regulated expression of miR 21 (Mean Cq 27.95 [+ or -] 1.63 Vs 26.51 [+ or -] 1.00) after 3 cycle of standard chemotherapy out of four tumor suppressor mir-200a, mir-200b, mir-205 and mir-145). This study had shown the all tumor suppressor miRNAs 205 showed higher expression with decrease in mean count of CSCs (CD44+/CD24-) in patients positively responding therapy. So, this and similar type of study may help in guiding more precise treatment of chemotherapy with gene therapy in near future.]]></abstract><pub>Springer</pub></addata></record> |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; SpringerNature Journals; PubMed Central |
| subjects | Breast cancer Cancer Chemotherapy MicroRNA Stem cells |
| title | Significance of Breast Cancer Stem Cell Marker and Tumor Suppressor miRNAs in Breast Carcinoma: A Step Toward Precision Medicine |
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