Significance of Breast Cancer Stem Cell Marker and Tumor Suppressor miRNAs in Breast Carcinoma: A Step Toward Precision Medicine

Breast cancer is a complex disease with heterogeneity and several studies have been conducted to explore differentially expressed miRNA in carcinogenesis. Tumor suppressor miRNAs (miR 200a, miR 200b, miR205 and miR 145) are involved in various signalling pathways and promote carcinogenesis and Cancer stem cells (CSCs) noticed as the driving force of tumorigenesis and metastases. Thus, my objective was to explore relationship of expressed miRNAs and Cancer Stem Cells in breast cancer patients before and after chemotherapy. 39 Breast Cancer samples were recruited after pathological approval and ethical clarification. miRNA was quantified on real-time PCR by using exiqon cDNA and Sybr green kit. CSCs (CD44+/CD24-) were characterized by using CD44 and CD24 antibodies on BD flow cytometer. Breast Cancer Stem Cell marker CD44+/CD24- were significantly reduced after three cycle of chemotherapy (Average% & Mean counts: 7.60% & 590 Vs 3.22% & 291). However, the highest frequency of cells with expression of CD44-/CD24+ were observed and remain almost unchanged after 3 cycle of chemotherapy (Average % & Mean counts: 33.68% & 23,953 Vs 32.63% & 21,648). The Breast cancer patients showed significant (p