The Association Oftnf-[alpha] Gene Polymorphism With Risk of Oral Cancer

BACKGROUND: Cancer is a multifactorial disease, resulting from complex interactions between genetic and environmental factors. Tumor necrosis factor alpha (TNF-?) is a pro-inflammatory cytokine, which plays a pivotal role in the pathogenesis of a variety of infectious and inflammatory diseases. Tumor necrosis factor alpha (TNF-?) gene polymorphism has been a potential determinant of susceptibility to various types of cancer. OBJECTIVE: To explore the association of TNF-? promoter (-G238) and (-G308A) gene polymorphisms with the susceptibility of oral cancer. METHODS: A total 300 patients with oral cancer and 300 healthy volunteers were genotyped for the TNF-?- 238G/A and-308G/A polymorphism. Polymorphism variants were examined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Genotype frequencies were evaluated by Chi-square test strength of associations by odds ratio with 95% confidence intervals. RESULTS: The genotypes of TNF-? (-G308A) were not significant differences in the genotype and allele frequencies of the TNF-? (-G308A) gene polymorphism between oral cancer patients and control groups (p: >0.05). The genotypes of TNF-? (-G238A) gene polymorphism were significant differences in the between oral cancer patients and control groups(OR: 23.15, CI: 1.32-7.53, p: 0.01). Moreover the allele G and A frequencies was also significantly deference in between case controls (OR: 3.07, CI: 1.29-7.28, p: