Studies on Methylglyoxal-Modified-IgG Under Normal and High Glucose
IgG (6.67 [micro]M) was transferred to assay tubes containing 5 mM/10 mM glucose and mixed. To this mixture 6.67 [micro]M MGO was rapidly added and incubated for 7 days at 37 0C in capped glass vials. The modification was studied by UV absorbance spectroscopy, fluorescence spectroscopy, far UV and n...
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| Veröffentlicht in: | Indian journal of clinical biochemistry 2022-05, Vol.32 (S1), p.S99 |
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| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
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| Zusammenfassung: | IgG (6.67 [micro]M) was transferred to assay tubes containing 5 mM/10 mM glucose and mixed. To this mixture 6.67 [micro]M MGO was rapidly added and incubated for 7 days at 37 0C in capped glass vials. The modification was studied by UV absorbance spectroscopy, fluorescence spectroscopy, far UV and near UV circular dichroism, thioflavin T fluorescence, Congo red binding, SEM, TEM, FTIR spectroscopy and LC-MS. |
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| ISSN: | 0970-1915 |