Study of Methylene Tetra Hydro Folate Reductase [MTHFR] Gene Polymorphism in Stroke

Blockage of small arteries in the brain causes serious disturbance of brain functions a condition called as stoke. Deficiency of MTHFR that causes impairment of the methionine metabolism leads to hyper homocysteinemia. This variant has become recognized as the most common genetic cause of MTHFR gene polymorphism. The aim was to study the mutation of MTHFR gene polymorphism in stroke patients and homocysteine levels and NO synthase levels in stroke patients. To study the oxidative stress and antioxidants mechanism in the stroke patients, 30 stroke patients with the help of neurology department were recruited as cases. Age matched normal healthy 30 were controls who were staff and students of Narayana Medical College, Nellore. MTHFR gene plymorphism studied by PCR and gel electrophorosis. Nitric Oxide synthase estimated by kinetic method. The results indicated prolonged vasoconstriction and increased oxidative stress. The gene polymorphism of MTHFR is one of the risk factor for the stroke. C677 T the MTHFR gene located on 1P 36. This study of MTHFR gene polymorphism might allow us to know the role of gene therapy for stroke patients.