Sanitary status of three Croatian native grapevine varieties s podrucja Dalmacije ukljucene u postupak klonske selekcije

The research of sanitary status and virus presence was conducted on three native Croatian grapevine varieties (Kujudžusa bijela, Zlatarica vrgorska and Trnjak crni) that are grown in Dalmatia region. The investigation was done on 492 vines from 31 vineyards. Vines were tested by ELISA (Enzyme-Linked Immunosorbent Assay) on four main economic viruses: arabis mosaic virus (ArMV), grapevine fanleaf virus (GFLV), grapevine leafroll-associated virus 1 and 3 (GLRaV-1, GLRaV-3) as a first step in clonal selection of these rare native grapevine varieties. Altogether, 40 vines were free of all tested viruses. The research confirms high rate of virus infection and eroded sanitary status of all investigated varieties. The above indicates necessity of implementation clonal and sanitary selection programme. Keywords: native grapevine varieties, ELISA, grapevine viruses, Dalmatia Istraživanje sanitarnog statusa provedeno je na tri autohtone hrvatske sorte vinove loze (Kujudžuša bijela, Zlatarica vrgorska i Trnjak crni) koje se uzgajaju u Dalmaciji. Istraživanjem je obuhvaceno ukupno 492 trsa iz 31 vinograda. Uzorci su podvrgnuti serološkom testu ELISA (Enzyme-Linked Immunosorbent Assay) za analizu na prisustvo cetiri gospodarski najvažnija virusa: virus mozaika lista vinove loze (ArMV), virus lepezastog lista vinove loze (GFLV), virus uvijenosti lista tip 1 i tip 3 (GLRaV-1, GLRaV-3). Od ukupnog broja prikupljenih uzoraka, njih 40 je bilo potpuno slobodno od prisutnosti istraživanih virusa. Ovo istraživanje potvrduje visok udio virusom zaraženih trsova te loš sanitarni status istraživanih sorata. Sve navedeno ukazuje na nužnost provedbe klonske i sanitarne selekcije. Kljucne rijeci: autohtone sorte vinove loze, ELISA, virusi vinove loze, Dalmacija