THE INFLUENCES OF LACTOBACILLUS CELL-FREE SUPERNATANTS ON GROWTH AND VIRULENCE PROPERTIES OF CAMPYLOBACTER JEJUNI IN HUMAN ADENOCARCINOMA HUCRE KULTURUNDE CAMPYLOBACTER JEJUNI'NIN UREME VE VIRULANS OZELLIKLERI UZERINE ETKILERI

Objective: Lactobacilli are the most commonly used probiotics. We examined the influence of cell-free supernatants (CFSs) of Lactobacillus acidophilus (La), L. fermentum (Lf), L. plantarum (Lp) and L. rhamnosus (Lr) on growth, adhesion and invasion of Campylobacter jejuni 81116 and RM1221 in human adenocarcinoma colon cells (HT-29). We also analyzed the influences of CFSs, C. jejuni and their combinations on HT-29 cell viability. Materials and Methods: Growth and adhesive-invasive bacteria counts were determined using the spectrophotometric method and colony counting method, respectively. We used methyl thiazolyl diphenyl-tetrazolium bromide (MTT) assay for detection of HT-29 cell viability. Results: During two and four hours of incubation, the growth of RM1221 was significantly decreased (p