Clinical and histopathological evaluation of the effects of platelet rich plasma, platelet poor plasma and topical serum physiologic treatment on wound healing caused by radiofrequency electrosurgery in rats/Sicanlarda radyofrekans elektrocerrahi ile olusturulmus yarada trombositten zengin plazma, trombositten fakir plazma ve topikal serum fizyolojik tedavisinin yara iyilesmesine etkisinin klinik ve histopatolojik olarak degerlendirilmesi

Background and Design: The aim of our study was to histopathologically evaluate the effects of platelet rich plasma (PRP) and platelet poor plasma (PPP) treatment in wound healing process in rats with partial thickness wound. Materials and Methods: Rats were divided into three groups as topical serum physiologic (SP) (group 1, n=9), PPP (group 2, n=9), and PRP (group 3, n=9). Wound model was created by radiofrequency-electrosurgery. Each group was treated once with SP, PRP and PPP. Four-millimeter punch biopsies were collected from each rat on the 4th, 7th, 14th and 21st days. One rat in each of the PRP and SP groups was lost before the study was completed. Results: In our study, we observed that PRP significantly increased granulation tissue formation and angiogenesis after the fourth day of treatment. PPP increased granulation tissue formation and angiogenesis to a lesser extent than PRP but to a greater extent compared to SP. An increase in fibroblast density was observed in PRP and PPP groups compared to that in SP group on the 7th, 14th and 21st days. Epidermal detachment was significantly increased in the PRP group compared to PPP group on the 4th day. On 7th and 14th days, a higher increase was observed in the PPP and PRP groups compared to SP group. On the 21stday, epidermal detachment was observed in all the three groups. In terms of collagen synthesis levels, a significant increase was observed in PPP group compared to SP group on the 4th day and a significant increase was observed in PRP group compared to PPP group on the 21st day. There was no significant difference between groups on the 7th and 14th days. Conclusion: In our study, we observed that both PRP and PPP stimulate and increase angiogenesis, reepithelization, granulation tissue and collagen synthesis compared to SP. We observed that there was a significant increase in angiogenesis and granulation tissue formation in PRP group compared to PPP group. Keywords: PRP, PPP, SP, wound healing, angiogenesis, granulation, fibrosis, epithelization Amac: Calismamizin amaci trombositten zengin plazma (PRP) ve trombositten fakir plazmanin (PPP) kismi kalinlikli yara olusturulmus ratlarda yara yeri iyilesmesinde olusturacagi etkilerin histopatolojik olarak degerlendirilmesidir. Gerec ve Yontem: Sicanlar, serum fizyolojik (SF) (grup 1, n=9), PPP (grup 2, n=9), PRP (grup 3, n=9) grubu olacak sekilde 3 gruba ayrildi. Radyofrekans-elektrocerrahi yontemi ile yara modeli olusturuldu. Her bir gruba SF, PR