Dual Nanofibrous Bioactive Coatings on TiZr Implants

The objective of this research was to obtain a dual coating with antimicrobial properties on TiZr implants. The metallic surfaces were modified with two biopolymers (poly(lactic acid) and polycaprolactone), the first deposited by dip coating and the second by electrospinning, in order to create a nanofibers type of coating with antibacterial and bioactive effect. The surface characteristics of the obtained bioactive coatings were evaluated by Fourier Transformed Infrared Spectroscopy, by scanning electron microscopy and by contact angle measurements. The electrochemical characterization of the coatings was performed in simulated body fluid. The metallic ion release from the coated implant materials was measured by inductively coupled plasma mass spectrometry. The in vitro antimicrobial properties of the coatings were studied using agar disc diffusion method and percentage inhibition of growth method for two bacterial strains—S. aureus and E. coli. The presence of silver nanoparticles presented high inhibition zone against gram negative bacteria like E. coli. Cell viability of MC3T3-E1 osteoblasts and cytoskeleton morphology, were tested in vitro for the biological evaluation. The results on in vitro cell response indicated good cell membrane integrity and viability for such nanofibrous bioactive coatings compared to the control substrate. These surface architectures design on implant materials holds promise for biomedical applications, presenting good antimicrobial properties and promote cell adhesion and proliferation.