A Bioinformatics Study on the Regulatory Network of Genes Related to Rat Liver Growth and Development

In this paper, we use the original data of gene chip of rat primary hepatocytes treated with acetaminophen (APAP) to deeply analyze the interference effect of APAP on the biological processes / pathways of hepatocytes. The NCBI's GEO (Gene Expression Omnibus) database was used to extract data from APAP-treated rat liver primary cell samples and analyze them using the GCBI online platform. The sample data was divided into an APAP treatment group (experimental group) and a distilled water treatment group (control group) for differential gene analysis, functional analysis and signal pathway analysis. The results of gene chip analysis showed that at different doses and different times of APAP treatment, the gene regulation of hepatocytes changed differently, and further analysis suggested that APAP interfered with biological processes, metabolism, and development. Pathways related to development mainly include angiogenesis, embryonic development, neural development and organ development, among which organ development and neural development are the most enriched. In vitro hepatocyte genomics analysis showed that in addition to metabolism, APAP can interfere with a variety of biological processes, including development-related biological function networks, suggesting that it may have organ development and neurodevelopmental toxicity, teratogenic effects and neural tube developmental toxicity is consistent. This study shows that in-depth analysis of the biological network of gene chip data is of great value for understanding the gene regulatory network. Key words: Acetaminophen Genomics, Primary Liver Cells, Biological Function, Regulatory Network