IN VITRO INVESTIGATION OF THE EFFECT OF STROMAL VASCULAR FRACTION ISOLATED BY MECHANICAL AND ENZYMATIC METHODS ON WOUND HEALING/ MEKANIK VE ENZIMATIK YONTEM ILE IZOLE EDILEN STROMAL VASKULER FRAKSIYONUN YARA IYILESMESI UZERINDEKI ETKISININ IN VITRO INCELENMESI

Objective: The role of Stromal Vascular Fraction (SVF) cell suspension obtained from adipose tissue in wound healing is gaining importance day by day. In this study, the role of SVF cell population, isolated by enzymatic and mechanical methods in wound healing, will be examined in vitro. Material and Method: An SVF cell cocktail was isolated by mechanical and enzymatic methods after adipose tissue was obtained from 10 different patients by the liposuction method (the adipose tissue was divided into two equal groups for enzymatic and mechanical methods). After the isolation process is done, cell viability and cell number were counted using flow cytometry, and cell proliferation rates were measured by WST-1 assay. The Wound Healing Scratch Assay test, which is used as an in vitro model for wound healing, was performed with cell cocktails obtained from both groups. After isolation, the level of expression of the collagen type 1 gene, known to be involved in wound healing, was measured in both groups. Results: While the SVF (E-SVF) cell cocktail obtained bythe enzymatic method was 1,52 * [10.sup.6]/ml ([+ or -]3,63, n=10, p=0,015), the number of SVF (M-SVF) cell cocktails obtained by mechanical method was 0,67 * [10.sup.6]/ml ([+ or -]1,69, n=10, p=0,015). No significant difference was observed between cell viability of SVF cell cocktails isolated by two different methods. The M-SVF cell cocktail had a 10% higher cell proliferation rate compared to E-SVF. Furthermore, M-SVF cell migration rates and Coll gene levels were found to be higher than E-SVF cells. Conclusion: Although the cell number of the M-SVF cell cocktail was less than the cell number of the E-SVF cell cocktail, the collagen type 1 gene level of the M-SVF cell cocktail was found to be higher, so the M-SVF cell cocktail had higher wound healing properties. Keywords: Stromal vascular fraction, wound healing, stem cell Amac: Yag dokusundan elde edilen Stromal Vaskuler Fraksiyon (SVF) hucre suspansiyonun yara iyilesmesindeki rolu gun gectikce onem kazanmaktadir. Bu calismada enzimatik ve mekanik yontemler ile izole edilen SVF hucre populasyonun yara iyilesmesindeki rolu incelenecektir. Gerec ve Yontem: 10 farkli hastadan yag alma yontemi ile elde edilen yag dokusu (esit miktarda iki farkli gruba bolundukten sonra) mekanik ve enzimatik yontemler kullanilarak SVF hucre kokteyli izole edilmistir. Izolasyon sonrasinda hucre canliligi ve hucre sayisi akis sitometrisi kullanilarak sayilmis, hucre prolifera