Pulmonary Fibrosis Model Induced by Repeated Intraperitoneal Administration of Paraquat in Mice

The purpose of this paper is to establish a mouse model of lung injury and pulmonary fibrosis induced by paraquat (PQ), to explore the mechanism of batrogen-induced pulmonary fibrosis induced by fbromycin, and to provide theoretical basis for its clinical application. A more ideal animal model is laid to lay the foundation. Methods Male C57BL/6J (C57) mice (n=30) aged 8-10 weeks were randomly divided into blank group, paraquat group, paraquat + bleomycin group, and Elisa. Methods The expressions of TGF-[beta]1, a-SMA, Smad3 and Smad2 in plasma were detected on the 1st, 3rd, 7th, 14th and 21st day after exposure. The paraquat group was intraperitoneally injected with PQ10mg/kg and injected five times. Three days of injection, paraquat + bleomycin group intratracheal injection of bleomycin 3mr / kg. On the 7th, 14th, and 28th day after successful modeling, 10 rats in each group were used to measure the general condition, body mass and pathological changes of lung tissue to calculate the lung coefficient, observe the histological changes of rats, and measure the lungs of rats. Expression of hydroxyproline, superoxide dismutase (SOD), malondialdehyde (MDA) and KL-6 mucin. The statistical data SPSS21.0 was used for statistical analysis of the collected data. The comparison between the two groups was performed by Mann-Whitney U test. Experiments have shown that PQ intraperitoneal injection and tracheal injection of BLM can cause lung injury and fibrosis model. By comparing the increase in body mass, mental status, pathological changes of lung tissue, and rate of formation at different time points in the control group, it was shown that repeated small doses of intraperitoneal injection of PQ were compared with conventional bleomycin intratracheal injection. The mold is more convenient and effective, and the success rate is higher. Bleomycin can induce paraquat-induced pulmonary fibrosis by inhibiting the expression of TGF-01, a-SMA and Smad3, Smad2 proteins, and repeated low-dose intraperitoneal injection of PQ is a more ideal modeling method. The method induced bymycin has a higher success rate. Key words: Paraquat Death, Low-Dose Intraperitoneal Administration, Pulmonary Fibrosis, Lung Injury