Analysis of Sigma-1 Receptor Binding Ability Under Emotional Stress and Upon Administration of the Anxiolytic Afobazole

Specific binding of the sigma-1 receptor (Sigma1R) ligand [ 3 H]-(+)-pentazocine was determined in the P2 and P3 fractions of brain homogenates from inbred BALB/c and C57BL/6 mice after emotional stress in the open-field (OF) test and outbred CD-1 mice after administration of the anxiolytic afobazol...

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Veröffentlicht in:Pharmaceutical chemistry journal 2019-11, Vol.53 (8), p.673-677
Hauptverfasser: Abramova, E. V., Voronin, M. V., Seredenin, S. B.
Format: Artikel
Sprache:eng
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Zusammenfassung:Specific binding of the sigma-1 receptor (Sigma1R) ligand [ 3 H]-(+)-pentazocine was determined in the P2 and P3 fractions of brain homogenates from inbred BALB/c and C57BL/6 mice after emotional stress in the open-field (OF) test and outbred CD-1 mice after administration of the anxiolytic afobazole. Exposure in the OF test increased specific binding of [ 3 H]-(+)-pentazocine to the P3 fraction of BALB/c mice brain homogenates as characterized by catatonia in the OF test and did not affect ligand binding in C57BL/6 mice with active behavior in the OF test. Afobazole at a dose of 5 mg/kg increased specific binding of [ 3 H]-(+)-pentazocine to the P2 fraction of outbred CD-1 mice brain homogenates. The results indicated that Sigma1R was involved in the formation mechanism of emotional stress responses and in afobazole pharmacodynamics.
ISSN:0091-150X
1573-9031
DOI:10.1007/s11094-019-02060-6