A NOVEL HERBAL EXTRACT MEDICINE FOR LIVER HEMOSTASIS: AN EXPERIMENTAL ANIMAL STUDY/ KARACIGER HEMOSTAZINDA YENI BIR BITKISEL ILAC: DENEYSEL BIR HAYVAN CALISMASI

Objective: Bleeding is one of the major complications of surgical procedures. Severe bleeding can be seen after liver injury. Various methods have been described in the literature for stopping liver bleeding such as compression, Pringle maneuver, and other hemostatic agents have been used. In this study, the use of anti-inflammatory, antioxidant, antimicrobial, analgesic, and anticoagulant agents described in the literature were evaluated for hemostatic efficacy following liver injuries. Materials and Methods: In this study 28 Wistar albino female / male rats from out-bred production were used. Animals were divided in 4 groups. After liver damage model creation, various herbal substances were applied to the liver. Hematocrit and platelet counts were measured. In addition, for histopathologic changes in the liver parenchyma, the rats were sacrificed, and the liver was resected. Results: The amount of bleeding (p:0.001) and the preoperative and postoperative hematocrit changes (p:0.009) were statistically significant between the groups. There was no significant difference in connective tissue growth (p:0.065) and necrosis (p:0.062). There were significant differences in inflammation and without karyorrhexis (p:0.003). Discussion: Histopathological examinations showed that the groups using active agents had more connective tissue and kary-orrhexis. Therefore these tissues could help to stop bleeding. Keywords: Liver bleeding, Herbal medicine, hemostatic agent Amaç: Kanama, cerrahi islemlerin ana komplikasyonlarindan biridir. Karaciger hasari sonrasi ciddi kanamalar görülebilir. Literatürde kompresyon, Pringle manevrasi ve diger hemostatik ajanlar gibi karaciger kanamasini durdurmak için çesitli yöntemler tanimlanmistir. Bu çalismada, literatürde anti-enflamatuar, antioksidan, antimik-robiyal, analjezik ve antikoagülan etkileri gösterilmis ajanlarin kullaniminin, karaciger yaralanmalarinda hemostatik etkinlikleri degerlendirildi. Gereç ve Yöntem: Bu çalismada 28 adet Wistar albino disi / erkek siçan kullanildi. Hayvanlar 4 gruba ayrildi. Karaciger hasari modeli olusturulduktan sonra, karacigere çesitli bitkisel karisimlar uygulandi. Hematokrit ve trombosit sayilari ölçüldü. Ayrica karaciger parankimindeki histopatolojik degisiklikler için, siçanlar sakrifiye edilerek karaciger rezeke edildi. Bulgular: Kanama miktari (p:0,001) ve preoperatif ve postoperatif hematokrit degisiklikleri (p:0,009) gruplar arasinda istatistiksel olarak anlamliydi. Bag dokusu büyümesi