TAURIN KIRIK IYILESMESINI ETKILER MI? DENEYSEL BIR CALISMA/DOES TAURINE IMPROVE FRACTURE HEALING? AN EXPERIMENTAL STUDY

Objective: To evaluate the influence of taurine, a non-structural amino acid with antioxidant effects, on fracture healing. Materials and Methods: Open tibial osteotomy was performed on nine New Zealand rabbits in the control group and seven in the taurine group. Radiologic fracture healing was assesed using the Goldberg score on x-rays and computed tomography (CT) sections on the 21st day. Healing was graded histologically using the Huo score. Serum malondialdehyde (MDA) and alkaline phosphatase (ALP) levels were measured, at days zero, seven, 14 and 21. Results: Upon completion of the study, in the taurine group, the median stage of healing was recorded as "radiologic union", whereas it was "probable union" in the control group with respect to x-ray and CT sections. In the taurine group, callus was mainly composed of osteoid tissue (median Huo score 8); in the control group the predominant tissue was chondroid (median Huo score 6). Changes in MDA levels revealed that oxidative stress was greatest in the inflammatory phase. In both groups, the ALP levels first decreased, and then increased ,with new bone formation on the 21st day. Conclusion: Taurine improved early bone healing in an experimental animal osteotomy model. This influence might be related to its antioxidant properties. Keywords: Taurine, fracture healing, oxidative stress, antioxidants, malondialdehyde (MDA) Amaç: Yapisal olmayan bir amino asit olan antioksidan etkili taurinin kirik iyilesmesi üzerine etkilerinin arastirilmasi Gereç ve Yöntem: Kontrol grubunda dokuz, taurin grubunda yedi tane Yeni Zelanda tipi tavsana açik tibial osteotomi uygulandi. Radyolojik kirik iyilesmesi, 21. günde çekilen direk grafi ve bilgisayarli tomografi (BT) kesitleri üzerinde Goldberg skoru ile degerlendirildi. Histoloik olarak iyilesme Huo skoruna göre derecelendirildi. Sifirinci, yedinci, 14. ve 21. günlerde serumda malondialdehit (MDA) ve alkalen fosfataz (ALP) düzeyleri ölçüldü. Bulgular: Direk grafi ve BT kesitlerine göre kirik iyilesmesinin ortanca evresi taurin grubunda radyolojik kaynamayken kontrol grubunda olasi kaynama olarak bulundu. Histolojik olarak, kallus taurin grubunda esas olarak kemik dokusundan olusurken (ortanca Huo skoru 8), kontrol grubunda baskin doku kikirdakti (ortanca Huo skoru 6). MDA düzeyindeki degisimler oksidatif stresin kirik iyilesmesinin enflamatuvar fazinda en yüksek oldugunu göstermekteydi. Her iki grupta da ALP düzeyleri önce azalmis, 21. Günde yeni kemik olusumuyla yenide