Alterations of the Giant Pyramidal Neurons in Brain Cortex of Rat Offspring Born from Gestational Diabetic Dams: A Morphometric Study/Alteraciones de las Neuronas Piramidales Gigantes de la Corteza Cerebral en Crias de Ratas Nacidas de Hembras con Diabetes Gestacional: Un Estudio Morfometrico

A few studies reported the adverse effects of gestational diabetes on hippocampus and spinal cord of rat offspring. Giant pyramidal neurons are giant pyramidal neurons located in fifth layers of the gray matter in the primary motor cortex. Therefore, this study was conducted to determine the effect of gestational diabetes on the giant pyramidal neurons and the thickness of internal pyramidal layer in the brain cortex of rat offspring. On day 1 of gestation, 10 Wistar rat dams were randomly allocated into two control and diabetic groups. Five animals in diabetic group received 40 mg/kg/BW of Streptozotocin (intraperitoneally) and control animals received normal saline. We randomly selected six offspring of every subject in both groups at day 28, 56 and 84. Rat offspring were scarified and then coronal sections were taken from the right brain cortex and sections were stained with Cresyl violet. The density of giant pyramidal neurons in brain cortex and thickness of internal pyramidal layer of brain cortex were evaluated. In P28, P56, P84 the Betz cells density of brain cortex were significantly reduced from 107.6 [+ or -] 6.2, 131.6 [+ or -] 4.6 and 143.5 [+ or -] 4.0 in controls to 84.96 [+ or -] 2.1, 109.8 [+ or -] 7.3 and 121.05 [+ or -] 5.6 in cases (p