[nu] and [beta]1 Integrins Mediate A[beta]-Induced Neurotoxicity in Hippocampal Neurons via the FAK Signaling Pathway

[alpha][nu] and [beta]1 integrins mediate A[beta]-induced neurotoxicity in primary hippocampal neurons. We treated hippocampal neurons with 2.5 [micro]g/mL 17E6 and 5 [micro]g/mL ab58524, which are specific [alpha][nu] and [beta]1 integrin antagonists, respectively, for 42 h prior to 10 [micro]M A[beta] treatment. Next, we employed small interfering RNA (siRNA) to silence focal adhesion kinase (FAK), a downstream target gene of integrins. The siRNAs were designed with a target sequence, an MOI of 10 and the addition of 5 [micro]g/mL polybrene. Under these conditions, the neurons were transfected and the apoptosis of different cell types was detected. Moreover, we used real-time PCR and Western blotting analyses to detect the expression of FAK and [rho]FAK genes in different cell types and investigated the underlying mechanism and signal pathway by which [alpha][nu] and [beta]1 integrins mediate A[beta]-induced neurotoxicity in hippocampal neurons. An MTT assay showed that both 17E6 and ab58524 significantly increased cell viability compared with the A[beta]-treated neurons (P