sup.1H-NMR-Based Metabolomic Profiling of CSF in Early Amyotrophic Lateral Sclerosis

Pathophysiological mechanisms involved in amyotrophic lateral sclerosis (ALS) are complex and none has identified reliable markers useful in routine patient evaluation. The aim of this study was to analyze the CSF of patients with ALS by .sup.1 H NMR (Nuclear Magnetic Resonance) spectroscopy in orde...

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Veröffentlicht in:PloS one 2010-10, Vol.5 (10), p.e13223
Hauptverfasser: Blasco, Hélène, Corcia, Philippe, Moreau, Caroline, Veau, Ségolène, Fournier, Clémentine, Vourc'h, Patrick, Emond, Patrick, Gordon, Paul, Pradat, Pierre-François, Praline, Julien, Devos, David, Nadal-Desbarats, Lydie, Andres, Christian R
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Sprache:eng
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Zusammenfassung:Pathophysiological mechanisms involved in amyotrophic lateral sclerosis (ALS) are complex and none has identified reliable markers useful in routine patient evaluation. The aim of this study was to analyze the CSF of patients with ALS by .sup.1 H NMR (Nuclear Magnetic Resonance) spectroscopy in order to identify biomarkers in the early stages of the disease, and to evaluate the biochemical factors involved in ALS. CSF samples were collected from patients with ALS at the time of diagnosis and from patients without neurodegenerative diseases. One and two-dimensional .sup.1 H NMR analyses were performed and metabolites were quantified by the ERETIC method. We compared the concentrations of CSF metabolites between both groups. Finally, we performed principal component (PCA) and discriminant analyses. CSF screening by NMR spectroscopy could be a useful, simple and low cost tool to improve the early diagnosis of ALS. The results indicate a perturbation of glucose metabolism, and the need to further explore cerebral energetic metabolism.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0013223