Isolation and identification of Mycobacterium bovis in milk from cows in northeastern Brazil/Isolamento e identificacao de Mycobacterium bovis em leite de vacas no Nordeste do Brasil

Milk samples from 16 cows that tested positive on the tuberculin test in the state of Paraiba, northeastern Brazil, were used for mycobacteria isolation and identification. Mycobacteria were isolated from five (31.25%) of the 16 milk samples; three samples were classified as M. bovis, and two as bel...

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Veröffentlicht in:Ciência rural 2016-12, Vol.46 (12), p.2166
Hauptverfasser: Ramos, Joelson Marcolino, Heinemann, Marcos Bryan, Neto, Jose Soares Ferreira, Filho, Antonio Francisco de Souza, Cardenas, Nicolas Cespedes, Alves, Clebert Jose, de Azevedo, Sergio Santos
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Sprache:spa
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Zusammenfassung:Milk samples from 16 cows that tested positive on the tuberculin test in the state of Paraiba, northeastern Brazil, were used for mycobacteria isolation and identification. Mycobacteria were isolated from five (31.25%) of the 16 milk samples; three samples were classified as M. bovis, and two as belonging to the Mycobacterium genus. This is probably the first study of isolation and identification of M. bovis in milk from cows in Northeastern Brazil, which suggests that humans are at risk of contamination by ingestion. Key words: Mycobacterium bovis, isolation, milk, PCR, cattle. Amostras de leite de 16 vacas positivas no teste da tuberculinizacao no Estado da Paraiba, Nordeste do Brasil, foram utilizadas para isolamento e identificacao de micobacterias. Foram isoladas micobacterias em cinco (31,25%) das 16 amostras de leite: tres amostras foram classificadas como M. bovis e duas como pertencentes ao genero Mycobacterium. De acordo com nosso conhecimento, este e o primeiro estudo de isolamento e identificacao de M. bovis em leite de vacas no Nordeste do Brasil, o que sugere que os seres humanos estao em risco de contaminacao por ingestao. Palavras-chave: Mycobacterium bovis, isolamento, leite, PCR, bovinos.
ISSN:0103-8478
DOI:10.1590/0103-8478cr20160416