Effect of genistein on basal jejunal chloride secretion in RII7H CF mice is sex and route specific

Cystic fibrosis (CF) results from the loss or reduction in function of the CFTR (cystic fibrosis transmembrane conductance regulatory protein) chloride channel. The third most common CFTR mutation seen clinically is R117H. Genistein, a naturally occurring phytoestrogen, is known to stimulate CFTR function in vitro. We aimed to determine whether route of administration of genistein could mediate differential effects in R117H male and female CF mice. Mice were fed (4 weeks) or injected subcutaneously (1 week) with the following: genistein 600 mg/kg diet (600Gd); genistein-free diet (0Gd); genistein injection 600 mg/kg body weight (600Gi); dimethyl sulfoxide control (0Gi). In male R117H mice fed 600Gd, basal short circuit current ([I.sub.sc]) was unchanged. In 600Gd-fed female mice, there was a subgroup that demonstrated a significant increase in basal [I.sub.sc] (53.14 ± 7.92 µA/[cm.sup.2], n=6, P