By Regulating Mitochondrial Ca.sup.2+-Uptake UCP2 Modulates Intracellular Ca.sup.2

By Regulating Mitochondrial Ca.sup.2+-Uptake UCP2 Modulates Intracellular Ca.sup.2

The possible role of UCP2 in modulating mitochondrial Ca.sup.2+ -uptake (mCa.sup.2+ -uptake) via the mitochondrial calcium uniporter (MCU) is highly controversial. Thus, we analyzed mCa.sup.2+ -uptake in isolated cardiac mitochondria, MCU single-channel activity in cardiac mitoplasts, dual Ca.sup.2+...

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Veröffentlicht in:PloS one 2016-02, Vol.11 (2)
Hauptverfasser: Motloch, Lukas Jaroslaw, Larbig, Robert, Gebing, Tina, Reda, Sara, Schwaiger, Astrid, Leitner, Johannes, Wolny, Martin, Eckardt, Lars, Hoppe, Uta C
Format: Artikel
Sprache:eng
Schlagworte:
Calcium ions
Genetic aspects
Mitochondria
Physiological aspects
Transport proteins
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Zusammenfassung:The possible role of UCP2 in modulating mitochondrial Ca.sup.2+ -uptake (mCa.sup.2+ -uptake) via the mitochondrial calcium uniporter (MCU) is highly controversial. Thus, we analyzed mCa.sup.2+ -uptake in isolated cardiac mitochondria, MCU single-channel activity in cardiac mitoplasts, dual Ca.sup.2+ -transients from mitochondrial ((Ca.sup.2+ )m) and intracellular compartment ((Ca.sup.2+ )c) in the whole-cell configuration in cardiomyocytes of wild-type (WT) and UCP2.sup.-/- mice. Isolated mitochondria showed a Ru360 sensitive mCa.sup.2+ -uptake, which was significantly decreased in UCP2.sup.-/- (229.4±30.8 FU vs. 146.3±23.4 FU, P
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0148359