Chlorophyllin in the intra-uterine development of mice exposed or not to cyclophosphamide/Clorofilina no desenvolvimento intra-uterino de camundongos expostos ou nao a ciclofosfamida

Chlorophyllin, a sodium-copper salt synthesized from chlorophyll, has already proved to have anticlastogenic, antimutagenic and anticarcinogenic activity, however few are the studies in the teratogenicity area. The present study evaluated the effects of chlorophyllin in intra-uterine development of mice exposed or not to cyclophosphamide. Pregnant females were divided into 8 groups of 15 animals each, G01 - PBS (0.1 mL 10.0-1 g) orally; G02 - cyclophosphamide (20.0 mg [kg.sup.-1]) i.p.; G03, G04 and G05 - chlorophyllin at concentrations of (5.0, 10.0 and 15.0 mg [kg.sup.-1]) orally; G06, G07 and G08 (5.0, 10.0 and 15.0 mg [kg.sup.-1]) orally, of chlorophyllin, respectively, and (20.0 mg [kg.sup.-1]) i.p. of cyclophosphamide. In the 18th day the females were submitted to laparotomy and females and fetuses analyzed. The results showed that the chlorophyllin was not effective in protecting the reproductive parameters as well as teratogenicity. Finally, it was observed that the presence of chlorophyllin increased the frequency of some malformations when combined with cyclophosphamide. However, it was not teratogenic and not embryo lethal in this experimental design. Keywords: antimutagenic, anticarcinogenic, food additives, pregnancy, teratogenesis. Clorofilina e um sal de cobre e sodio sintetizado a partir da clorofila. Provou-se ter atividade anticlastogenica, antimutagenica e anticarcinogenica. No entanto, poucos sao os estudos sobre esta substancia na area de teratologia. Dessa forma, o presente trabalho avaliou os efeitos da clorofilina no desenvolvimento intrauterino de camundongos expostos ou nao a ciclofosfamida. Para tal, femeas prenhez foram divididas em oito grupos experimentais contendo 15 animais cada: G01 - PBS (0,1 mL 10.0-1 g) via oral; G02 - ciclofosfamida (20,0 mg [kg.sup.-1]), intraperitoneal; G03, G04 e G05 - clorofilina em concentracoes de (5,0; 10,0 e 15,0 mg [kg.sup.-1]) via oral; G06, G07 e G08 (5,0, 10,0 e 15,0 mg [kg.sup.-1]) via oral, de clorofilina, respectivamente, e 20,0 mg [kg.sup.-1], via intraperitoneal, de ciclofosfamida. No 18 dia de gestacao, os animais foram submetidos a laparotomia e os fetos, analisados para parametros teratogenicos. Os resultados mostraram que a clorofilina nao foi eficaz para proteger os parametros reprodutivos, bem como a teratogenicidade. Finalmente, foi observado que a clorofilina quando combinada com a ciclofosfamida aumentou a frequencia de algumas malformacoes. No entanto, a clorofilina nao se apr