Goat spermatozoa after freezing in skimmed-milk extender supplemented with reduced glutathione/Espermatozoides caprinos criopreservados em meio a base de leite desnatado acrescido de glutationa reduzida

Aiming to evaluate in vitro effect of different concentrations of glutathione reduced (GSH) in skimmed-milk and glycerol 7°% it was used semen from five Boer bucks. After collect and evaluation, a pool of samples was diluted in skimmed-milk and glycerol 7% plus antioxidant: G1) Control; G2) GSH 2mM [mL.sup.-1]; G3) GSH 5mM [mL.sup.-1] and G4) GSH 7mM [mL.sup.-1]. Samples were frozen in straws (0.25mL) and stored at 1960C. After thawing, samples were subjected to integrity of the plasma membrane (iMP) and acrosomal (iAc), mitochondrial membrane potential (MMP), kinematic and ultrastructure analysis. Control and GSH (2, 5 and 7mM [mL.sup.-1]) groups did no differ (P>0.05) in iMP, iAc, PMM and kinematic parameters. In the ultrastructural analysis, percentages of acrosome and plasma membrane (tail and head region) intact did not differ (P>0.05) between groups. However, Control group had higher percentage (P0,05) entre grupos. Todavia, o grupo Controle apresentou maior porcentual (P