Light in intermediate acclimatization of in vitro germinated seedlings of Dendrobium phalaenopsis Deang Suree/Luz na aclimatizacao intermediaria de mudas germinadas in vitro de Dendrobium phalaenopsis Deang Suree

The success in micropropagation of Dendrobium phalaenopsis Deang Suree is high, but when transplanted into the greenhouse, their survival is minimal. To increase survival in production in the present study it was evaluated the effect of intermediate acclimatization for 30 days in a grow room utilizing the following luminosity conditions: 1--white fluorescent light (B) (18.9µmol [m.sup.-2] [s.sup.-1]); 2--white fluorescent light + red fluorescent light (GRO-LUX®) (BV) (14.85µmol [m.sup.-2] [s.sup.-1]); 3--red fluorescent light (GRO-LUX®) (V) (9.45µmol [m.sup.-2] [s.sup.-1]) and the control plants were accommodated directly in a greenhouse (162.0µmol [m.sup.-2] [s.sup.-1]). After this the leaves were characterized anatomically and the plants transferred to the control greenhouse. It was evaluated survival percentage and final number of roots, and calculated the relations between the final and initial values of fresh weight, number of leaves, length and diameter of the largest pseudo bulb, number of pseudo bulbs and longest root length. Only plants submitted to red light, were statistically better than the control in relation to the survival percentage and in relation to fresh weight, while the control showed a higher number of roots that plants acclimatized in this luminosity conditions. Intermediate acclimatization, using red light or red + white light, is recommended for D. phalaenopsis Deang Suree. Key words: Orchidaceae, floriculture, luminosity conditions, in vitro cultivation. O sucesso na micropropagacao de Dendrobium phalaenopsis Deang Suree e alto, porem, quando transplantado para o viveiro, sua sobrevivencia e minima. Com o intuito de aumentar a sobrevivencia na producao, no presente trabalho, avaliou-se o efeito da aclimatizacao intermediaria, por 30 dias em sala de crescimento, sob as seguintes condicoes de luminosidade: 'luz fluorescente branca (18,9µmol [m.sup.-2] [s.sup.-1]); 2--luz fluorescente branca + luz fluorescente vermelha (14,85µmol [m.sup.-2] [s.sup.-1]); e 3--luz fluorescente vermelha (9,45µmol [m.sup.-2] [s.sup.-1]), o controle foi acondicionado diretamente em viveiro coberto (162,0µmol [m.sup.-2] [s.sup.-1]). Na sequencia, as folhas foram caracterizadas anatomicamente e as plantas foram transferidas para o viveiro que continha o controle. Foi avaliada a porcentagem de sobrevivencia e o numero final de raizes, sendo calculadas tambem as relacoes entre os valores finais e iniciais de massa fresca, numero de folhas, comprimento e diam