Leaf tissues proportion and chemical composition of Axonopus jesuiticus x A. scoparius as a function of pig slurry application/Proporcao de tecidos foliares e composicao quimica de Axonopus jesuiticus x A. scoparius em funcao da aplicacao de dejeto liquido de suino

This study aimed to evaluate the chemical and anatomical attributes of leaves of giant missionary grass to application of 0, 62, 124, 186, 248 and 310[m.sup.3] [ha.sup.-1] of pig slurry. At 83 days after the last application of fertilizer, the leaf blades were collected, fixed in FAA 70%, sectioned, stained, photographed and digitalized. The transversal section of leaf blades were evaluated for proportion of epidermis, lignified vascular tissue + sclerenchyma, non-lignified vascular tissue and parenchyma with an image-processing system calibrated to 1mm [pixel.sup.-1]. Leaf samples were analyzed for crude protein, acid detergent fiber, neutral detergent fiber and hemicellulose content by near infrared reflectance spectroscopy. The pig slurry application up to 310[m.sup.3] [ha.sup.-1] significantly increased the percentage of crude protein, parenchyma, epidermis, non-lignified vascular tissue and hemicellulose, while decreasing the percentage of acid detergent fiber and lignified vascular tissue + sclerenchyma. The Pearson's correlation was positive between crude protein and non-lignified vascular tissue, and between acid detergent fiber and lignified vascular tissue + sclerenchyma. The percentage of hemicellulose was positively correlated with epidermis, parenchyma and nonlignified vascular tissue. A negative correlation between acid detergent fiber and epidermis, parenchyma and non-lignified vascular tissue was observed. Key words: crude protein, epidermis, fiber, lignified vascular tissue + sclerenchyma, parenchyma. Este trabalho teve como objetivo avaliar os atributos anatomicos e quimicos da grama-missioneira-gigante a aplicacao de 0, 62, 124, 186, 248 and 310[m.sup.3] [ha.sup.-1]. Decorridos 83 dias do ultimo corte e da aplicacao do dejeto, as laminas foliares foram coletadas, fixadas em FAA 70%, seccionadas, coradas, fotografadas e digitalizadas. Seccoes transversais das laminas foliares foram avaliadas quanto a proporcao das areas de epiderme, tecido vascular lignificado + esclerenquima, tecido vascular nao lignificado e parenquima, na regiao entre a quilha e o segundo maior feixe vascular, com sistema de processamento de imagem calibrado para 1mm [pixel.sup.-1]. Amostras de folhas foram coletadas e analisadas quanto ao teor de proteina bruta, fibra insoluvel em detergente acido e fibra insoluvel em detergente neutro, pelo metodo de espectrometria de reflectancia no infravermelho proximal. A aplicacao de ate 310[m.sup.3] [ha.sup.-1] do dejeto liquid