ω-conotoxin MVIIA intralesional injection in spinal cord injury in rats/Aplicacao intralesional da ω-conotoxina MVIIA no trauma medular em ratos
This study aimed to investigate the neuroprotective effect of ω-conotoxin MVIIA (MVIIA) intralesional application in rats submitted to spinal cord injury Male Wistar rats, weighing 300g ± 23.4, were distributed in five groups: negative control (SHAM), placebo (PLA), 5µM MVIIA, 10µM MVIIA and 20µM MV...
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| creator | de Oliveira, Karen Maciel Binda, Nancy Scardua Lavor, Mario Sergio Lima Silva, Carla Maria Osorio Rosado, Isabel Rodrigues Taguchi, Tatiana Malagoli Alves, Endrigo Gabellini Leonel Melo, Marilia Ma Gomez, Marcus Vinicius de Melo, Eliane Goncalves |
| description | This study aimed to investigate the neuroprotective effect of ω-conotoxin MVIIA (MVIIA) intralesional application in rats submitted to spinal cord injury Male Wistar rats, weighing 300g ± 23.4, were distributed in five groups: negative control (SHAM), placebo (PLA), 5µM MVIIA, 10µM MVIIA and 20µM MVIIA MVIIA. After laminectomy of the 12th thoracic vertebra (SHAM), the PLA, 5µM MVIIA, 10µM MVIIA and 20µM MVIIA groups were subjected to acute compressive spinal cord trauma for five minutes, and then five minutes later, the animals received specific treatment in a standard total volume of 2µL, by intralesional route, using sterile PBS as placebo. Locomotor activity was assayed using Basso Beattie Bresnahan (BBB) scale to show the patterning of SCI. With 48 hours of injury, the animals were euthanized, the liquor sample was collected in atlantooccipital space, and also the spinal segment, including the epicenter and caudal region to injury. Assays were performed for mitochondrial viability, serum glutamate, production of reactive oxygen species (ROS) and lipid peroxidation (LP) were performed. The study design was randomized and the data submitted to ANOVA and comparison of means by SNK test, and data from BBB scale were evaluated using Kruskal-Wallis test (P |
| doi_str_mv | 10.1590/0103-8478cr20141203 |
| format | Article |
| fullrecord | <record><control><sourceid>gale</sourceid><recordid>TN_cdi_gale_infotracmisc_A441766718</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A441766718</galeid><sourcerecordid>A441766718</sourcerecordid><originalsourceid>FETCH-LOGICAL-g678-c65796cfbd555a554d72a89c060f935ba77b85700d72f6769f747d738531edce3</originalsourceid><addsrcrecordid>eNptj0tLAzEQx3NQsFY_gZeA522TzWOyx6X4KFS8FK8lzaOk7G7KZgv6Ebz6xfxKZrGIiMxhZn7znxdCN5TMqKjInFDCCsVBmb4klNOSsDM0-aEX6DKlPSElMM4n6OPzvTCxi0N8DR1-elkuaxy6odeNSyF2usnZ3pkhxznC6RBGZmJvx8Kxfxtpr4c0rw9NMNro-Kffavx7hz4t6SLOqmOrcevssdE9du04KKYrdO51k9z1yU_R-v5uvXgsVs8Py0W9KnYSVGGkgEoav7VCCC0Et1BqVRkiia-Y2GqArRJASOZegqw8cLDAlGDUWePYFN1-j93lWzeh8zHfY9qQzKbmnIKUQFVWzf5RZbOuDfkp50Pmvxq-AKZqeDE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>ω-conotoxin MVIIA intralesional injection in spinal cord injury in rats/Aplicacao intralesional da ω-conotoxina MVIIA no trauma medular em ratos</title><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>de Oliveira, Karen Maciel ; Binda, Nancy Scardua ; Lavor, Mario Sergio Lima ; Silva, Carla Maria Osorio ; Rosado, Isabel Rodrigues ; Taguchi, Tatiana Malagoli ; Alves, Endrigo Gabellini Leonel ; Melo, Marilia Ma ; Gomez, Marcus Vinicius ; de Melo, Eliane Goncalves</creator><creatorcontrib>de Oliveira, Karen Maciel ; Binda, Nancy Scardua ; Lavor, Mario Sergio Lima ; Silva, Carla Maria Osorio ; Rosado, Isabel Rodrigues ; Taguchi, Tatiana Malagoli ; Alves, Endrigo Gabellini Leonel ; Melo, Marilia Ma ; Gomez, Marcus Vinicius ; de Melo, Eliane Goncalves</creatorcontrib><description>This study aimed to investigate the neuroprotective effect of ω-conotoxin MVIIA (MVIIA) intralesional application in rats submitted to spinal cord injury Male Wistar rats, weighing 300g ± 23.4, were distributed in five groups: negative control (SHAM), placebo (PLA), 5µM MVIIA, 10µM MVIIA and 20µM MVIIA MVIIA. After laminectomy of the 12th thoracic vertebra (SHAM), the PLA, 5µM MVIIA, 10µM MVIIA and 20µM MVIIA groups were subjected to acute compressive spinal cord trauma for five minutes, and then five minutes later, the animals received specific treatment in a standard total volume of 2µL, by intralesional route, using sterile PBS as placebo. Locomotor activity was assayed using Basso Beattie Bresnahan (BBB) scale to show the patterning of SCI. With 48 hours of injury, the animals were euthanized, the liquor sample was collected in atlantooccipital space, and also the spinal segment, including the epicenter and caudal region to injury. Assays were performed for mitochondrial viability, serum glutamate, production of reactive oxygen species (ROS) and lipid peroxidation (LP) were performed. The study design was randomized and the data submitted to ANOVA and comparison of means by SNK test, and data from BBB scale were evaluated using Kruskal-Wallis test (P<0.05). There was no significant difference between groups in BBB scores. The MVIIA did not promote decrease in the levels of glutamate, ROS, LP, and did not preserve the mitochondria in the intralesional application five minutes after spinal cord injury in rats. Key words: MVIIA, cone snail, cell viability, glutamate, reactive oxygen species, lipid peroxidation. Objetivou-se investigar o efeito neuroprotetor da aplicacao intralesional da MVIIA em ratos submetidos ao trauma medular. Foram utilizados ratos Wistar, machos, com peso entre 300g ± 23.4, distribuidos em cinco grupos: controle negativo (SHAM), placebo (PLA), 5µM MVIIA, 10µM MVIIA e 20µM MVIIA. Apos a laminectomia da vertebra toracica 12 (SHAM), os grupos PLA, 5µM MVIIA, 10µM MVIIA e 20µM MVIIA foram submetidos ao trauma medular agudo compressivo por cinco minutos e, cinco minutos apos o trauma, receberam o tratamento especifico em volume total padrao de 2µL, pela via intralesional, sendo utilizado como placebo o PBS esteril. A atividade locomotora foi avaliada pela escala proposta por Basso Beattie Bresnahan (BBB), com intuito de mostrar a padronizacao do trauma medular. Com 48 horas do trauma, os animais foram submetidos a eutanasia, coletou-se amostra do liquor no espaco atlantooccipital e um segmento medular, incluindo o epicentro e regiao caudal a lesao. Foram realizados ensaios de viabilidade mitocondrial, dosagem de glutamato, producao de especies reativas de oxigenio (ERO) e peroxidacao lipidica (PL). O delineamento do estudo foi inteiramente casualizado e os dados submetidos ao ANOVA, com comparacao de medias pelo teste de SNK e os dados do teste BBB foram comparados utilizando o teste Kruskal-Wallis (P<0.05). Em relacao aos escores do BBB, nao houve diferenca entre os grupos. A MVIIA nao promoveu a diminuicao dos niveis do glutamato, ERO, PL e nao preservou a mitocondria na aplicacao intralesional, cinco minutos apos o trauma medular em ratos. Palavras-chave: MVIIA, caramujo marinho, viabilidade celular, glutamato, especies reativas de oxigenio, peroxidacao lipidica.</description><identifier>ISSN: 0103-8478</identifier><identifier>DOI: 10.1590/0103-8478cr20141203</identifier><language>spa</language><publisher>Universidade Federal de Santa Maria</publisher><ispartof>Ciência rural, 2016-01, p.150</ispartof><rights>COPYRIGHT 2016 Universidade Federal de Santa Maria</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,861,27905,27906</link.rule.ids></links><search><creatorcontrib>de Oliveira, Karen Maciel</creatorcontrib><creatorcontrib>Binda, Nancy Scardua</creatorcontrib><creatorcontrib>Lavor, Mario Sergio Lima</creatorcontrib><creatorcontrib>Silva, Carla Maria Osorio</creatorcontrib><creatorcontrib>Rosado, Isabel Rodrigues</creatorcontrib><creatorcontrib>Taguchi, Tatiana Malagoli</creatorcontrib><creatorcontrib>Alves, Endrigo Gabellini Leonel</creatorcontrib><creatorcontrib>Melo, Marilia Ma</creatorcontrib><creatorcontrib>Gomez, Marcus Vinicius</creatorcontrib><creatorcontrib>de Melo, Eliane Goncalves</creatorcontrib><title>ω-conotoxin MVIIA intralesional injection in spinal cord injury in rats/Aplicacao intralesional da ω-conotoxina MVIIA no trauma medular em ratos</title><title>Ciência rural</title><description>This study aimed to investigate the neuroprotective effect of ω-conotoxin MVIIA (MVIIA) intralesional application in rats submitted to spinal cord injury Male Wistar rats, weighing 300g ± 23.4, were distributed in five groups: negative control (SHAM), placebo (PLA), 5µM MVIIA, 10µM MVIIA and 20µM MVIIA MVIIA. After laminectomy of the 12th thoracic vertebra (SHAM), the PLA, 5µM MVIIA, 10µM MVIIA and 20µM MVIIA groups were subjected to acute compressive spinal cord trauma for five minutes, and then five minutes later, the animals received specific treatment in a standard total volume of 2µL, by intralesional route, using sterile PBS as placebo. Locomotor activity was assayed using Basso Beattie Bresnahan (BBB) scale to show the patterning of SCI. With 48 hours of injury, the animals were euthanized, the liquor sample was collected in atlantooccipital space, and also the spinal segment, including the epicenter and caudal region to injury. Assays were performed for mitochondrial viability, serum glutamate, production of reactive oxygen species (ROS) and lipid peroxidation (LP) were performed. The study design was randomized and the data submitted to ANOVA and comparison of means by SNK test, and data from BBB scale were evaluated using Kruskal-Wallis test (P<0.05). There was no significant difference between groups in BBB scores. The MVIIA did not promote decrease in the levels of glutamate, ROS, LP, and did not preserve the mitochondria in the intralesional application five minutes after spinal cord injury in rats. Key words: MVIIA, cone snail, cell viability, glutamate, reactive oxygen species, lipid peroxidation. Objetivou-se investigar o efeito neuroprotetor da aplicacao intralesional da MVIIA em ratos submetidos ao trauma medular. Foram utilizados ratos Wistar, machos, com peso entre 300g ± 23.4, distribuidos em cinco grupos: controle negativo (SHAM), placebo (PLA), 5µM MVIIA, 10µM MVIIA e 20µM MVIIA. Apos a laminectomia da vertebra toracica 12 (SHAM), os grupos PLA, 5µM MVIIA, 10µM MVIIA e 20µM MVIIA foram submetidos ao trauma medular agudo compressivo por cinco minutos e, cinco minutos apos o trauma, receberam o tratamento especifico em volume total padrao de 2µL, pela via intralesional, sendo utilizado como placebo o PBS esteril. A atividade locomotora foi avaliada pela escala proposta por Basso Beattie Bresnahan (BBB), com intuito de mostrar a padronizacao do trauma medular. Com 48 horas do trauma, os animais foram submetidos a eutanasia, coletou-se amostra do liquor no espaco atlantooccipital e um segmento medular, incluindo o epicentro e regiao caudal a lesao. Foram realizados ensaios de viabilidade mitocondrial, dosagem de glutamato, producao de especies reativas de oxigenio (ERO) e peroxidacao lipidica (PL). O delineamento do estudo foi inteiramente casualizado e os dados submetidos ao ANOVA, com comparacao de medias pelo teste de SNK e os dados do teste BBB foram comparados utilizando o teste Kruskal-Wallis (P<0.05). Em relacao aos escores do BBB, nao houve diferenca entre os grupos. A MVIIA nao promoveu a diminuicao dos niveis do glutamato, ERO, PL e nao preservou a mitocondria na aplicacao intralesional, cinco minutos apos o trauma medular em ratos. Palavras-chave: MVIIA, caramujo marinho, viabilidade celular, glutamato, especies reativas de oxigenio, peroxidacao lipidica.</description><issn>0103-8478</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNptj0tLAzEQx3NQsFY_gZeA522TzWOyx6X4KFS8FK8lzaOk7G7KZgv6Ebz6xfxKZrGIiMxhZn7znxdCN5TMqKjInFDCCsVBmb4klNOSsDM0-aEX6DKlPSElMM4n6OPzvTCxi0N8DR1-elkuaxy6odeNSyF2usnZ3pkhxznC6RBGZmJvx8Kxfxtpr4c0rw9NMNro-Kffavx7hz4t6SLOqmOrcevssdE9du04KKYrdO51k9z1yU_R-v5uvXgsVs8Py0W9KnYSVGGkgEoav7VCCC0Et1BqVRkiia-Y2GqArRJASOZegqw8cLDAlGDUWePYFN1-j93lWzeh8zHfY9qQzKbmnIKUQFVWzf5RZbOuDfkp50Pmvxq-AKZqeDE</recordid><startdate>20160101</startdate><enddate>20160101</enddate><creator>de Oliveira, Karen Maciel</creator><creator>Binda, Nancy Scardua</creator><creator>Lavor, Mario Sergio Lima</creator><creator>Silva, Carla Maria Osorio</creator><creator>Rosado, Isabel Rodrigues</creator><creator>Taguchi, Tatiana Malagoli</creator><creator>Alves, Endrigo Gabellini Leonel</creator><creator>Melo, Marilia Ma</creator><creator>Gomez, Marcus Vinicius</creator><creator>de Melo, Eliane Goncalves</creator><general>Universidade Federal de Santa Maria</general><scope>INF</scope></search><sort><creationdate>20160101</creationdate><title>ω-conotoxin MVIIA intralesional injection in spinal cord injury in rats/Aplicacao intralesional da ω-conotoxina MVIIA no trauma medular em ratos</title><author>de Oliveira, Karen Maciel ; Binda, Nancy Scardua ; Lavor, Mario Sergio Lima ; Silva, Carla Maria Osorio ; Rosado, Isabel Rodrigues ; Taguchi, Tatiana Malagoli ; Alves, Endrigo Gabellini Leonel ; Melo, Marilia Ma ; Gomez, Marcus Vinicius ; de Melo, Eliane Goncalves</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g678-c65796cfbd555a554d72a89c060f935ba77b85700d72f6769f747d738531edce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>spa</language><creationdate>2016</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de Oliveira, Karen Maciel</creatorcontrib><creatorcontrib>Binda, Nancy Scardua</creatorcontrib><creatorcontrib>Lavor, Mario Sergio Lima</creatorcontrib><creatorcontrib>Silva, Carla Maria Osorio</creatorcontrib><creatorcontrib>Rosado, Isabel Rodrigues</creatorcontrib><creatorcontrib>Taguchi, Tatiana Malagoli</creatorcontrib><creatorcontrib>Alves, Endrigo Gabellini Leonel</creatorcontrib><creatorcontrib>Melo, Marilia Ma</creatorcontrib><creatorcontrib>Gomez, Marcus Vinicius</creatorcontrib><creatorcontrib>de Melo, Eliane Goncalves</creatorcontrib><collection>Gale OneFile: Informe Academico</collection><jtitle>Ciência rural</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>de Oliveira, Karen Maciel</au><au>Binda, Nancy Scardua</au><au>Lavor, Mario Sergio Lima</au><au>Silva, Carla Maria Osorio</au><au>Rosado, Isabel Rodrigues</au><au>Taguchi, Tatiana Malagoli</au><au>Alves, Endrigo Gabellini Leonel</au><au>Melo, Marilia Ma</au><au>Gomez, Marcus Vinicius</au><au>de Melo, Eliane Goncalves</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>ω-conotoxin MVIIA intralesional injection in spinal cord injury in rats/Aplicacao intralesional da ω-conotoxina MVIIA no trauma medular em ratos</atitle><jtitle>Ciência rural</jtitle><date>2016-01-01</date><risdate>2016</risdate><spage>150</spage><pages>150-</pages><issn>0103-8478</issn><abstract>This study aimed to investigate the neuroprotective effect of ω-conotoxin MVIIA (MVIIA) intralesional application in rats submitted to spinal cord injury Male Wistar rats, weighing 300g ± 23.4, were distributed in five groups: negative control (SHAM), placebo (PLA), 5µM MVIIA, 10µM MVIIA and 20µM MVIIA MVIIA. After laminectomy of the 12th thoracic vertebra (SHAM), the PLA, 5µM MVIIA, 10µM MVIIA and 20µM MVIIA groups were subjected to acute compressive spinal cord trauma for five minutes, and then five minutes later, the animals received specific treatment in a standard total volume of 2µL, by intralesional route, using sterile PBS as placebo. Locomotor activity was assayed using Basso Beattie Bresnahan (BBB) scale to show the patterning of SCI. With 48 hours of injury, the animals were euthanized, the liquor sample was collected in atlantooccipital space, and also the spinal segment, including the epicenter and caudal region to injury. Assays were performed for mitochondrial viability, serum glutamate, production of reactive oxygen species (ROS) and lipid peroxidation (LP) were performed. The study design was randomized and the data submitted to ANOVA and comparison of means by SNK test, and data from BBB scale were evaluated using Kruskal-Wallis test (P<0.05). There was no significant difference between groups in BBB scores. The MVIIA did not promote decrease in the levels of glutamate, ROS, LP, and did not preserve the mitochondria in the intralesional application five minutes after spinal cord injury in rats. Key words: MVIIA, cone snail, cell viability, glutamate, reactive oxygen species, lipid peroxidation. Objetivou-se investigar o efeito neuroprotetor da aplicacao intralesional da MVIIA em ratos submetidos ao trauma medular. Foram utilizados ratos Wistar, machos, com peso entre 300g ± 23.4, distribuidos em cinco grupos: controle negativo (SHAM), placebo (PLA), 5µM MVIIA, 10µM MVIIA e 20µM MVIIA. Apos a laminectomia da vertebra toracica 12 (SHAM), os grupos PLA, 5µM MVIIA, 10µM MVIIA e 20µM MVIIA foram submetidos ao trauma medular agudo compressivo por cinco minutos e, cinco minutos apos o trauma, receberam o tratamento especifico em volume total padrao de 2µL, pela via intralesional, sendo utilizado como placebo o PBS esteril. A atividade locomotora foi avaliada pela escala proposta por Basso Beattie Bresnahan (BBB), com intuito de mostrar a padronizacao do trauma medular. Com 48 horas do trauma, os animais foram submetidos a eutanasia, coletou-se amostra do liquor no espaco atlantooccipital e um segmento medular, incluindo o epicentro e regiao caudal a lesao. Foram realizados ensaios de viabilidade mitocondrial, dosagem de glutamato, producao de especies reativas de oxigenio (ERO) e peroxidacao lipidica (PL). O delineamento do estudo foi inteiramente casualizado e os dados submetidos ao ANOVA, com comparacao de medias pelo teste de SNK e os dados do teste BBB foram comparados utilizando o teste Kruskal-Wallis (P<0.05). Em relacao aos escores do BBB, nao houve diferenca entre os grupos. A MVIIA nao promoveu a diminuicao dos niveis do glutamato, ERO, PL e nao preservou a mitocondria na aplicacao intralesional, cinco minutos apos o trauma medular em ratos. Palavras-chave: MVIIA, caramujo marinho, viabilidade celular, glutamato, especies reativas de oxigenio, peroxidacao lipidica.</abstract><pub>Universidade Federal de Santa Maria</pub><doi>10.1590/0103-8478cr20141203</doi></addata></record> |
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| title | ω-conotoxin MVIIA intralesional injection in spinal cord injury in rats/Aplicacao intralesional da ω-conotoxina MVIIA no trauma medular em ratos |
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