ω-conotoxin MVIIA intralesional injection in spinal cord injury in rats/Aplicacao intralesional da ω-conotoxina MVIIA no trauma medular em ratos

This study aimed to investigate the neuroprotective effect of ω-conotoxin MVIIA (MVIIA) intralesional application in rats submitted to spinal cord injury Male Wistar rats, weighing 300g ± 23.4, were distributed in five groups: negative control (SHAM), placebo (PLA), 5µM MVIIA, 10µM MVIIA and 20µM MVIIA MVIIA. After laminectomy of the 12th thoracic vertebra (SHAM), the PLA, 5µM MVIIA, 10µM MVIIA and 20µM MVIIA groups were subjected to acute compressive spinal cord trauma for five minutes, and then five minutes later, the animals received specific treatment in a standard total volume of 2µL, by intralesional route, using sterile PBS as placebo. Locomotor activity was assayed using Basso Beattie Bresnahan (BBB) scale to show the patterning of SCI. With 48 hours of injury, the animals were euthanized, the liquor sample was collected in atlantooccipital space, and also the spinal segment, including the epicenter and caudal region to injury. Assays were performed for mitochondrial viability, serum glutamate, production of reactive oxygen species (ROS) and lipid peroxidation (LP) were performed. The study design was randomized and the data submitted to ANOVA and comparison of means by SNK test, and data from BBB scale were evaluated using Kruskal-Wallis test (P