Anatomical variations of Laelia purpurata var. carnea in vitro cultured under different intensities and spectral quality of light/Variacoes anatomicas de Laelia purpurata var. carnea cultivada in vitro sob diferentes intensidades e qualidade spectral de luz

In vitro propagated orchid (Laelia purpurata var. carnea) were maintained in a growth chamber in a greenhouse and subjected to different spectral intensities and quality of light, using different colored shade-net (blue, red and black). It was observed that the light intensity and spectral quality i...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Ciência rural 2012-03, Vol.42 (3), p.480
Hauptverfasser: da Silva, Jr., Jesse Marques, de Castro, Evaristo Mauro, Rodrigues, Marcelo, Pasqual, Moacir, Bertolucci, Suzan Kelly Vilela
Format: Artikel
Sprache:spa
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:In vitro propagated orchid (Laelia purpurata var. carnea) were maintained in a growth chamber in a greenhouse and subjected to different spectral intensities and quality of light, using different colored shade-net (blue, red and black). It was observed that the light intensity and spectral quality influenced significantly all the parameters analyzed at 120 days of cultivation. Plants cultivated under blue color shade net at growth chamber and greenhouse presented higher thickening of epidermis on the adaxial face (31.73 and 35.46pm), respectively, and were statistically different when compared with other treatments. It was observed a significant increase in the number of velamen cell layers in plant roots grown in the greenhouse, regardless of the color shade-net used when compared with plants cultivated at growth chamber. It was observed a higher differentiation of tissues (canopy, exodermis, cortex and vascular cylinder) in cross sections plant roots grown in greenhouse under blue shade-net. Key words: orchid, in vitro propagation, colored shade-nets, acclimatization. Plantas de Laelia purpurata var. carnea propagadas in vitro e mantidas em sala de crescimento e em casa de vegetacao foram submetidas a diferentes intensidades e qualidade espectral de luz, envolvidas com diferentes malhas coloridas: azul, vermelha e preta. Foi observado que a intensidade e qualidade espectral de luz interferiram de forma significativa em todos os parametros analisados, aos 120 dias de cultivo. Plantas cultivadas sob malha azul em sala de crescimento e casa de vegetacao apresentaram maior espessamento da epiderme na face adaxial (31,73 e 35,46pm), respectivamente, diferindo estatisticamente quando comparado com os demais tratamentos. Foi observado aumento significativo no numero de camadas de celulas do velame em raizes de plantas cultivadas em casa de vegetacao, independente da malha de sombreamento, quando comparadas com as de sala de crescimento. Em seccoes transversais de raizes de plantas cultivadas em casa de vegetacao sob malha azul, foi observada maior diferenciacao dos tecidos (velame, exoderme, cortex e cilindro vascular). Palavras-chave: orquidea, propagacao in vitro, malhas fotoconversoras, aclimatizacao.
ISSN:0103-8478