Comparison between Oligoryzomys nigripes and O. flavescens by RAPD and genetic diversity in O. nigripes por RAPD

The genus of Oligoryzomys includes species of small size, morphologically similar, which may impede taxonomic identification, mainly between O. flavescens (Waterhouse, 1837) and O. nigripes (Olfers, 1818). The main objective of this work was to investigate whether the RAPD markers are capable of genetically differentiating the specimens O. nigripes and O. flavescens, coming from Rio Grande do Sul (RS) and Santa Catarina (SC) states, and also to estimate the genetic variability among populations of O. nigripes, with the Uruguay River as a geographical barrier. For this purpose, samples were collected in fragments of forests situated in the North of RS, at FLONA (Floresta Nacional de Passo Fundo) and in fragments from SC, close to the Uruguay River. The karyotyping of two samples for each species was carried out and compared using the RAPD technique together with non- karyotyped individuals. Samples of O. nigripes presented 2n = 62; NA = 82, with submetacentric arms on the largest chromosomes, while samples of O. flavescens showed 2n = 64; NA = 66, with the largest chromosomes presenting acrocentric morphology, making such a result the main difference between the species. The analysis was able to detect two distinct groups, being the first one with karyotyped O. flavescens and the second with karyotyped O. nigripes. Identification afforded 211 loci, among them 181 (85.78%) polymorphic. The Jaccard similarity coefficient was in the range of 0.45 to 0.87. The UPGMA and Main Coordinate Analysis techniques demonstrated the existence of heterogeneous genetics among populations, but did not separate them completely in terms of geographical standards, and they are not influenced by the Uruguay River, which did not act as an efficient barrier. Keywords: cytogenetics, RAPD, Oligoryzomys,molecular markers. O genero Oligoryzomys inclui especies de tamanho pequeno, morfologicamente semelhantes, com dificil identificacao taxonomica, principalmente entre O. flavescens e O. nigripes. O objetivo principal deste trabalho foi investigar se os marcadores RAPD sao capazes de diferenciar geneticamente as amostras de O. nigripes e O. flavescens, oriundas do Rio Grande do Sul (RS) e Santa Catarina (SC) e tambem para estimar o variabilidade genetica entre populacoes de O. nigripes, tendo o rio Uruguai como uma barreira geografica. Para este fim, as amostras foram coletadas em fragmentos florestais situados no Norte do RS, na FLONA (Floresta Nacional de Passo Fundo) e em fragmentos