Hematology parameters in dogs treated with subconjunctival application of limbal stem cells allografts/Vrijednosti hematoloskih pokazatelja u pasa lijecenih supkonjunktivalnom primjenom stanicnog alografta

The cornea, as a source for obtaining stem cells, is a good medium for their use in veterinary ophthalmology. In this research, we used dogs which had been diagnosed with extensive, chronic thinning of the central cornea (vertex corneae) generated due to complicated mechanical damage to the corneae pannosa. The goal of this study was to test whether subconjuctival application of limbal stem cells allografts had any influence on haematology parameters in peripheral blood. The animals were divided into three experimental groups regardless of breed, sex and age, and each group comprised 7 dogs. Group A consisted of clinically healthy dogs (control), in group B there were dogs who had had injuries treated by a conventional ophthalmological approach in veterinary medicine, and group C in which corneal injuries were treated using subconjuctival cell allografts. Blood samples were taken four times during the experiment from all animals for haematological analysis. Transplantation of limbal stem cell allografts did not affect the physiological haematology parameters. Key words: dog, stem cell, limbus, veterinary ophthalmology Roznica, kao izvor maticnih stanica, dobar je medij za njihovu uporabu u veterinarskoj oftalmologiji. U ovom istrazivanju koristili smo pse kojima su dijagnosticirana opsezna, kronicna stanjivanja sredisnjeg dijela roznice, posljedica mehanickih ostecenja, dodatno komplicirana upalnim procesima. Cilj istrazivanja bio je ispitati utjecaj supkonjunktivalne primjene limbalnog stanicnog alografta na hematoloske pokazatelje u perifernoj krvi pacijenata. Zivotinje su bile podijeljene u tri pokusne skupine, bez obzira na pasminu, spol i dob. U svakoj skupini bilo je sedam pasa. U skupini A bili su klinicki zdravi psi (kontrolna skupina), u skupini B bili su psi kojima su ozljede obradene konvencionalnim oftalmoloskim pristupom, a psima u skupini C ostecenja roznice bila su lijecena supkonjunktivalnom primjenom stanicnog alografta. Cetiri puta tijekom istrazivanja svim su zivotinjama uzeti uzorci periferne venske krvi za hematoloske pretrage. Supkonjunktivalna primjena stanicnog alografta nije utjecala na promjenu vrijednosti hematoloskih pokazatelja u obradivanih pasa. Kljucne rijeci: pas, maticne stanice, limbus, veterinarska oftalmologija