Use of the bubaline acellular diaphragm matrix for umbilical hernioplasty in pigs/Upotreba bezstanicne matrice iz osita bivola pri pupcanoj hernioplastici svinja

Acellularization of xenogenic biomaterials has been highly desired for implantation without considerable adverse inflammatory and immune responses. Therefore, the present study was undertaken to acellularize the diaphragm of buffalo origin and to evaluate the efficacy of this prepared acellular diaphragm matrix (ADM) scaffolds for the repair of umbilical hernia in pigs. Buffalo diaphragms were acellularized with different concentrations of sodium deoxycholate over a range of periods. Produced ADM scaffolds were used during umbilical hernioplasty in 12 crossbred Landrace pigs. Harvested serum samples on post-implantation days (0, 15 and 30) were subjected to sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) to observe serum protein distribution pattern. Treatment with 2 % sodium deoxycholate lead to complete acellularization of the bubaline diaphragm at 48 h. Microscopic examination of the detergent treated matrix confirmed complete extraction of stromal cells and orderly arranged collagen fibres. All the hernias repaired with ADM remained sound over a period of 3 months. The serum protein distribution on post-implantation day 15 was found different from day zero and becomes normal on day 30. All the hernias implanted with ADM healed completely without graft rejections. The present study suggested that 2 % sodium deoxycholate can be used for complete acellularization of bubaline diaphragm, and produced ADM may be used safely for umbilical hernioplasty in pigs. Key words: acellular diaphragm matrix, buffalo, pigs, SDS-PAGE, umbilical hernia Bezstanicna struktura ksenogenih biomaterijala smatra se povoljnom jer pri implantaciji ne uzrokuje upalne i imunoloske reakcije. U ovom je istrazivanju proveden postupak dobivanja bezstanicne matrice iz osita (BMO) bivola koji je zatim bio rabljen kao podloga za obnavljanje tkiva pri operaciji pupcane hernije svinja. Gubitak stanicne strukture bivoljih osita proveden je uz pomoc razlicitih koncentracija natrijeva deoksikolata, primijenjenih u odredenim vremenskim razmacima. Proizvedene bezstanicne matrice osita upotrijebljene su pri pupcanoj hernioplastici kod 12 svinja krizanaca landras pasmine. Uzorci seruma, prikupljeni 0., 15. i 30. dana nakon implantacije, bili su podvrgnuti natrijev dodecil sulfat-poliakrilamid gel elektroforezi (SDS-PAGE) s ciljem promatranja razdiobe serumskih proteina. Obrada 2 %-tnim natrijevim deoksikolatom dovela je do potpunog gubitka stanicne strukture bivoljeg osita za