Immunophenotyping of dendritic cells of the residing porcine gut-associated lymphoid tissues/Imunofenotipizacija dendriticnih stanica u organiziranim limfnim tkivima probavnog sustava svinja

The aim of the study was to analyze the distribution patterns of porcine dendritic cells (DCs) of the residing mucosa of the ileum, caecum and colon, as well as mesenteric/colonic lymph nodes (MLN/CLN) of adult pigs. Three-color immunofluorescence (IF) was applied using monoclonal antibodies specific for porcine cell surface molecules (CD172, CD11R1 and CD16) on DCs. DCs were strategically localized within the lamina propria (LP) of the ileal villi, adjacent to the epithelial layer and around the crypts of the caecum/colon. Within the ileal LP, [CD16.sup.+] and [CD11R1.sup.+] cells were prevalent, whereas [CD172.sup.+] cells were much rarer. Conversely, in the caecum the latter cells were more prominent, the [CD16.sup.+] cells were also numerous, while [CD11R1.sup.+] cells were extremely rare. In the colonic mucosa the most frequent were [CD16.sup.+].[CD11R1.sup.+] cells were very rare, whereas [CD172.sup.+] cells were almost absent. In the MLN, [CD172.sup.+] cells were prevalent within the follicular area, [CD16.sup.+] cells were rare and none of the [CD11R1.sup.+] cells were examined. Within the perifollicular area the most numerous were [CD16.sup.+] cells, [CD11R1.sup.+] cells were less prevalent, while [CD172.sup.+] cells were sporadic. In the CLN, [CD16.sup.+] cells were the most frequent in the perifollicular area, [CD11R1.sup.+] cells were less frequent and none of the [CD172.sup.+] cells were observed. Within the follicular area the most numerous were [CD11R1.sup.+] cells, [CD172.sup.+] cells were very rare and [CD16.sup.+] cells were absent. Key words: dendritic cells, gut-associated lymphoid tissues, pig Cilj istrazivanja bio je odrediti razmjestaj svinjskih dendriticnih stanica (DS) u sluznici ileuma, cekuma i kolona te u mezenterijskim limfnim cvorovima (MLC) i limfnim cvorovima kolona (LCK) odraslih svinja. Trobojna imunofl uorescencijska (IF) pretraga koristena je uz upotrebu monoklonskih protutijela specifi cnih za svinjske povrsinske molekule (CD172, CD11R1, MHC II i CD16) na dendriticne stanice. DS su strateski lokalizirane unutar lamine proprije (LP) crijevnih resica, neposredno uz epitelni sloj te uokolo kripti cekuma i kolona. Unutar LP ileuma prevladavale su [CD16.sup.+] i [CD11R1.sup.+] stanice, dok su [CD172.sup.+] stanice bile mnogo rijede. Nasuprot tome, u cekumu je bila izrazenija prisutnost [CD172.sup.+] stanica, dok su [CD16.sup.+] stanice bile takoder brojne, a [CD11R1.sup.+] stanice izrazito rijetke. U mukozi kolona najcesce s