QuEChERS coupled to dispersive liquid-liquid microextraction for the determination of aflatoxins B1 and M1 in dairy foods by HPLC

s A rapid method is proposed for determining aflatoxins B1 and M1 in milk and dairy products by HPLC with fluorimetric detection. To extract aflatoxins from samples and purify the extracts of impurities the QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) method was used. The purified extracts were preconcentrated by dispersive liquid-liquid microextraction. The detection limits for aflatoxins B1 and M1 are 0.1 and 0.01 μg/kg, respectively. The relative standard deviation of the results of analysis is no more than 6%. The duration of analysis is 1–1.5 h.