Magnetic separation as a new method for the extraction of small molecules from biological fluids of humans
A new version of extraction is proposed for the extraction of doping substances from urine. It is based on magnetic separation using ferromagnetic microparticles with the surface modified with C 18 groups. A procedure is developed on its basis for the quantification of selective modulators of androg...
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| Veröffentlicht in: | Journal of analytical chemistry (New York, N.Y.) N.Y.), 2011-09, Vol.66 (9), p.807-814 |
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| container_end_page | 814 |
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| container_issue | 9 |
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| container_title | Journal of analytical chemistry (New York, N.Y.) |
| container_volume | 66 |
| creator | Sukhanova, I. I. Dikunets, M. A. Viryus, E. D. Rodchenkov, G. M. |
| description | A new version of extraction is proposed for the extraction of doping substances from urine. It is based on magnetic separation using ferromagnetic microparticles with the surface modified with C
18
groups. A procedure is developed on its basis for the quantification of selective modulators of androgen receptors and peroxisome proliferator-activated delta-receptor agonists using high-performance liquid chromatography coupled with tandem mass spectrometry. Using the proposed approach, the recovery of doping substances from human biological fluids approximates 100%, while the negative effect of the matrix components on the ionization of target compounds is reduced to a minimum. In contrast to solid phase extraction and liquid-liquid extraction, the method of magnetic separation appears to be more rapid and simple. The time of pretreatment for a sample intended for HPLC coupled to tandem mass spectrometry makes 5 min; the detection limit makes 0.25–5 ng/mL. |
| doi_str_mv | 10.1134/S1061934811090164 |
| format | Article |
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18
groups. A procedure is developed on its basis for the quantification of selective modulators of androgen receptors and peroxisome proliferator-activated delta-receptor agonists using high-performance liquid chromatography coupled with tandem mass spectrometry. Using the proposed approach, the recovery of doping substances from human biological fluids approximates 100%, while the negative effect of the matrix components on the ionization of target compounds is reduced to a minimum. In contrast to solid phase extraction and liquid-liquid extraction, the method of magnetic separation appears to be more rapid and simple. The time of pretreatment for a sample intended for HPLC coupled to tandem mass spectrometry makes 5 min; the detection limit makes 0.25–5 ng/mL.</description><identifier>ISSN: 1061-9348</identifier><identifier>EISSN: 1608-3199</identifier><identifier>DOI: 10.1134/S1061934811090164</identifier><language>eng</language><publisher>Dordrecht: SP MAIK Nauka/Interperiodica</publisher><subject>Analytical Chemistry ; Chemistry ; Chemistry and Materials Science ; Ferromagnetism ; Ionization ; Liquid chromatography ; Mass spectrometry ; Methods</subject><ispartof>Journal of analytical chemistry (New York, N.Y.), 2011-09, Vol.66 (9), p.807-814</ispartof><rights>Pleiades Publishing, Ltd. 2011</rights><rights>COPYRIGHT 2011 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c427t-b8d3556c113ae79ff0fb8ff65f4b3bd3e395ee74f350dd732fb5f21bf96768393</citedby><cites>FETCH-LOGICAL-c427t-b8d3556c113ae79ff0fb8ff65f4b3bd3e395ee74f350dd732fb5f21bf96768393</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S1061934811090164$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S1061934811090164$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Sukhanova, I. I.</creatorcontrib><creatorcontrib>Dikunets, M. A.</creatorcontrib><creatorcontrib>Viryus, E. D.</creatorcontrib><creatorcontrib>Rodchenkov, G. M.</creatorcontrib><title>Magnetic separation as a new method for the extraction of small molecules from biological fluids of humans</title><title>Journal of analytical chemistry (New York, N.Y.)</title><addtitle>J Anal Chem</addtitle><description>A new version of extraction is proposed for the extraction of doping substances from urine. It is based on magnetic separation using ferromagnetic microparticles with the surface modified with C
18
groups. A procedure is developed on its basis for the quantification of selective modulators of androgen receptors and peroxisome proliferator-activated delta-receptor agonists using high-performance liquid chromatography coupled with tandem mass spectrometry. Using the proposed approach, the recovery of doping substances from human biological fluids approximates 100%, while the negative effect of the matrix components on the ionization of target compounds is reduced to a minimum. In contrast to solid phase extraction and liquid-liquid extraction, the method of magnetic separation appears to be more rapid and simple. The time of pretreatment for a sample intended for HPLC coupled to tandem mass spectrometry makes 5 min; the detection limit makes 0.25–5 ng/mL.</description><subject>Analytical Chemistry</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Ferromagnetism</subject><subject>Ionization</subject><subject>Liquid chromatography</subject><subject>Mass spectrometry</subject><subject>Methods</subject><issn>1061-9348</issn><issn>1608-3199</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNp9kcFq3DAQQE1JoUnaD-hN0FMP3kiWLVvHZWmShQ2Bpj0bWZ7xapGlINk0-fvI3V6WLkGHEZr3Bs1Mln1ldMUYL2-eGBVM8rJhjErKRPkhu2SCNjlnUl6ke0rnS_5TdhXjgVIqGyYus8ODGhxMRpMIzyqoyXhHVCSKOPhDRpj2vifoA5n2QOBlCkr_RTySOCpryegt6NlCJBj8SDrjrR-MVpagnU0fF3I_j8rFz9lHVDbCl3_xOvt9--PX5j7fPd5tN-tdrsuinvKu6XlVCZ3aUlBLRIpdgygqLDve9Ry4rADqEnlF-77mBXYVFqxDKWrRcMmvs2_HuoOy0BqHfvn1aKJu17yqpRB1USQqP0MN4CAo6x2gSc8n_OoMn04Po9Fnhe8nQmKmNMBBzTG226efpyw7sjr4GANg-xzMqMJry2i77Lf9b7_JKY5OTKwbILQHPweXJvuO9AaIv6Ym</recordid><startdate>20110901</startdate><enddate>20110901</enddate><creator>Sukhanova, I. I.</creator><creator>Dikunets, M. A.</creator><creator>Viryus, E. D.</creator><creator>Rodchenkov, G. M.</creator><general>SP MAIK Nauka/Interperiodica</general><general>Springer</general><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope></search><sort><creationdate>20110901</creationdate><title>Magnetic separation as a new method for the extraction of small molecules from biological fluids of humans</title><author>Sukhanova, I. I. ; Dikunets, M. A. ; Viryus, E. D. ; Rodchenkov, G. M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c427t-b8d3556c113ae79ff0fb8ff65f4b3bd3e395ee74f350dd732fb5f21bf96768393</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Analytical Chemistry</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Ferromagnetism</topic><topic>Ionization</topic><topic>Liquid chromatography</topic><topic>Mass spectrometry</topic><topic>Methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sukhanova, I. I.</creatorcontrib><creatorcontrib>Dikunets, M. A.</creatorcontrib><creatorcontrib>Viryus, E. D.</creatorcontrib><creatorcontrib>Rodchenkov, G. M.</creatorcontrib><collection>CrossRef</collection><collection>Gale In Context: Science</collection><jtitle>Journal of analytical chemistry (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sukhanova, I. I.</au><au>Dikunets, M. A.</au><au>Viryus, E. D.</au><au>Rodchenkov, G. M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Magnetic separation as a new method for the extraction of small molecules from biological fluids of humans</atitle><jtitle>Journal of analytical chemistry (New York, N.Y.)</jtitle><stitle>J Anal Chem</stitle><date>2011-09-01</date><risdate>2011</risdate><volume>66</volume><issue>9</issue><spage>807</spage><epage>814</epage><pages>807-814</pages><issn>1061-9348</issn><eissn>1608-3199</eissn><abstract>A new version of extraction is proposed for the extraction of doping substances from urine. It is based on magnetic separation using ferromagnetic microparticles with the surface modified with C
18
groups. A procedure is developed on its basis for the quantification of selective modulators of androgen receptors and peroxisome proliferator-activated delta-receptor agonists using high-performance liquid chromatography coupled with tandem mass spectrometry. Using the proposed approach, the recovery of doping substances from human biological fluids approximates 100%, while the negative effect of the matrix components on the ionization of target compounds is reduced to a minimum. In contrast to solid phase extraction and liquid-liquid extraction, the method of magnetic separation appears to be more rapid and simple. The time of pretreatment for a sample intended for HPLC coupled to tandem mass spectrometry makes 5 min; the detection limit makes 0.25–5 ng/mL.</abstract><cop>Dordrecht</cop><pub>SP MAIK Nauka/Interperiodica</pub><doi>10.1134/S1061934811090164</doi><tpages>8</tpages></addata></record> |
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| ispartof | Journal of analytical chemistry (New York, N.Y.), 2011-09, Vol.66 (9), p.807-814 |
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| language | eng |
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| subjects | Analytical Chemistry Chemistry Chemistry and Materials Science Ferromagnetism Ionization Liquid chromatography Mass spectrometry Methods |
| title | Magnetic separation as a new method for the extraction of small molecules from biological fluids of humans |
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