Magnetic separation as a new method for the extraction of small molecules from biological fluids of humans
A new version of extraction is proposed for the extraction of doping substances from urine. It is based on magnetic separation using ferromagnetic microparticles with the surface modified with C 18 groups. A procedure is developed on its basis for the quantification of selective modulators of androg...
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Veröffentlicht in: | Journal of analytical chemistry (New York, N.Y.) N.Y.), 2011-09, Vol.66 (9), p.807-814 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | A new version of extraction is proposed for the extraction of doping substances from urine. It is based on magnetic separation using ferromagnetic microparticles with the surface modified with C
18
groups. A procedure is developed on its basis for the quantification of selective modulators of androgen receptors and peroxisome proliferator-activated delta-receptor agonists using high-performance liquid chromatography coupled with tandem mass spectrometry. Using the proposed approach, the recovery of doping substances from human biological fluids approximates 100%, while the negative effect of the matrix components on the ionization of target compounds is reduced to a minimum. In contrast to solid phase extraction and liquid-liquid extraction, the method of magnetic separation appears to be more rapid and simple. The time of pretreatment for a sample intended for HPLC coupled to tandem mass spectrometry makes 5 min; the detection limit makes 0.25–5 ng/mL. |
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ISSN: | 1061-9348 1608-3199 |
DOI: | 10.1134/S1061934811090164 |