Separation of four bioactive compounds from Herba artemisiae scopariae by HPLC with ionic liquid-based silica column

Commercial silica was modified with an amino-imidazolium ionic liquid and the obtained particles were used as a special sorbent in HPLC column to separate chlorogenic acid, caffeic acid, scoparone and rutin from Herba artemisiae scopariae . Acetonitrile/water (50 : 50, v/v) and acetonitrile/water (50 : 50, v/v, containing 0.1% triethylamine) mixtures were used as mobile phases for two-stage elutions. Good linearity were obtained from 0.2 × 10 −4 to 0.1 mg/mL ( r 2 > 0.998) with the relative standard deviations less than 5.2%.