Molecular identification of S-alleles of gametophytic incompatibility in Prunus salicina Lindl./Caracterizacao molecular dos alelo-S de incompatibilidade gametofitica em Prunus salicina Lindl

Commercial plum orchards must contain at least two cultivars, in order to ensure good fruit set and thus high economic yields, because its carries the gametophytic incompatibility system, that inhibits the self-pollination in a great number of cultivars. The aim of this work was to identify by PCR (Polymerase Chain Reaction) the S-alleles related to gametophytic incompatibility in eleven Prunus salicina (Lindl.) cultivars. The Santa Rosa, Santa Rita, Reubennel, Pluma 7, America, Rosa Mineira, Amarelinha, The First, Gulfblaze (Clone Sao Paulo), Gulfblaze (Clone Guaiba) e Harry Pickstone cultivars were analysed using three pairs of specific primers for amplifying the S-alleles. The PCR conditions and primers set allowed a characterization of S-alleles in the japonese plum and the identification of the most compatible pollinators to some commercial cultivars. Sequencing analysis of some amplified S-alleles showed high similarity to nucleotides sequences already identified in other studies with Prunus spp.. However, complete sequences of a large number of S-alleles it is necessary to get a more precisely identity of relationship among this S-alleles.