Effects of the fructooligosaccharides e inulina no metabolismo osseo de ratas esqueleticamente maduras

The aim of this study was establish if the fructooligosaccharides (FOS) and inulin alone or together attenuate age related bone loss in skeletally mature female rats. Forty 10-month old female rats were randomly assigned to four diet groups for 2 months: control, FOS, inulin and FOS + inulin. Bone mineral density (BMD) using dualenergy X-ray absorptiometry (DXA); femur quality using morphometry and biomechanic properties; biochemical assays by the determination of serum parathyroid hormone (PTH), alkaline phosphatase activity (ALP), degradation products of C-terminal peptides of type I collagen (CTX-I), osteocalcin (OC), osteoprotegerin (OPG) and nuclear factor Kappa B ligand (RANk-L). The FOS increased hip axis BMD (0.255 [+ or -] 0.005 g/[cm.sup.2]) and femur neck width (2.19 [+ or -] 0.01 mm) and reduced PTH (4.0 x [10.sup.-3] [+ or -] 0.0006 [micro]g/L); FOS + inulin increased the femur proportional limit (87.2 [+ or -] 1.0 N) and reduced PTH (2.5 x [10.sup.-3] [+ or -] 0.0006 [micro]g/L) and ALP (23.2 [+ or -] 5.1 U/L); all the prebiotics reduced OPG (FOS = 1.1 [+ or -] 0.3; inulin = 1.1 [+ or -] 0.3; FOS + inulin = 1.4 [+ or -] 0.4 [micro]g/L) and RANk-L (FOS = 1.65 x [10.sup.-2] [+ or -] 0.003; inulin = 1.78 x [10.sup.-2] [+ or -] 0.003; FOS + inulin = 2.83 x [10.sup.-2] [+ or -] 0.006 [micro]g/L); no prebiotics changed OC and CTX-I. The results suggested that the consumption of FOS or FOS + inulin may reduce the bone turnover, however, further studies about prebiotics and their synergistic effect on age related bone loss are required. KEYWORDS: Aging; bone tissue; prebiotics; osteoporosis; rats. O objetivo deste estudo foi estabelecer se os fructooligossacarideos (FOS) e a inulina sozinhos ou em conjunto atenuam a perda ossea relacionada a idade em ratas esqueleticamente maduras. Quarenta ratas com 10 meses de idade foram distribuidas aleatoriamente em 4 grupos por 2 meses: controle, FOS, inulina e FOS + inulina. A densidade mineral ossea (DMO) foi analisada usando absorcao de raios-X de dupla energia; a qualidade do femur foi analisada pelas propriedades biomecanicas e parametros morfometricos; os ensaios bioquimicos foram determinados pelo hormonio da paratireoide (PTH), atividade da fosfatase alcalina (ALP), produtos de degradacao de peptideos C-terminal de colageno tipo I (CTX-I), osteocalcina (OC), osteoprotegerina (OPG) e fator nuclear Kappa B ligante (RANk-L). O FOS aumentou a DMO do hip axis (0.255 [+ or -] 0.005 g/[cm.sup.2]) e do pescoco