The identity confirmation of [sup.99g]Tc-DMSA complexes by using NMR and HPLC-MS/MS methods

The analyses of [sup.99g]Tc-DMSA complexes prepared under alkali and acidic reactions were reported. Modern analytical, separation and spectral methods such as NMR (¹H-NMR, [sup.13]C-NMR, APT, COSY and HSQC) and Q-TOF HPLC-MS/MS system with ESI were employed to determine the identity and characteriz...

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Veröffentlicht in:Journal of radioanalytical and nuclear chemistry 2013-03, Vol.295 (3), p.2163
Hauptverfasser: Stanik, R, Benkovsky, I, Svetlik, J, Galba, J, Pronayova, N, Karlovska, J
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Sprache:eng
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Zusammenfassung:The analyses of [sup.99g]Tc-DMSA complexes prepared under alkali and acidic reactions were reported. Modern analytical, separation and spectral methods such as NMR (¹H-NMR, [sup.13]C-NMR, APT, COSY and HSQC) and Q-TOF HPLC-MS/MS system with ESI were employed to determine the identity and characterization of the products. The structure of [sup.99g]Tc(V)DMSA was clearly confirmed and its fragmentation path in negative and positive ionisation mode was suggested. The effect of ascorbic acid and new alternative labelling with the use of N[H.sup.99g.sub.4]TcO[Cl.sub.4] was examined. Surprisingly, [sup.99g]Tc(III)DMSA complex was not formed under acidic reaction conditions. [sup.99g]Tc(V)DMSA complex was the main reaction product under both experimental conditions. This result suggests the key role of [sup.99g/99m]Tc concentration during the process of radiopharmaceuticals preparation. Keywords [sup.99g]Tc-DMSA * NMR * HPLC-MS/MS
ISSN:0236-5731
DOI:10.1007/s10967-012-2254-4