In vitro biofilm activity of Candida species isolated from Anatolian buffaloes with mastitis in Western Turkey/In vitro aktivnost biofi lma vrsta roda Candida izdvojenih iz anatolijskih bivolica s mastitisom u zapadnoj Turskoj

In the present study, identification of 66 Candida isolates obtained from mastitic Anatolian buffalo quarter milk samples was achieved using the API 20 C AUX system. Among the isolates, the most common species were Candida krusei (27.3%), followed by Candida rugosa (16.7%), Candida kefyr (12.1%) and Candida tropicalis (10.6%). The other species were detected as Candida albicans (9.1%), Candida zeylanoides (6.1%), Candida parapsilosis (6.1%), Candida guilliermondii (4.5%), Candida famata (3.0%), Candida glabrata (3.0%) and Candida ciferrii (1.5%). A total of 53 (80.3%) Candida isolates produced the biofilm. All C. krusei, C. tropicalis, C. parapsilosis and C. guilliermondii strains were determined as biofilm positive. However, C. ciferrii did not produce any biofilm. This is the first study to investigate in vitro biofilm activity of different Candida species isolated from Anatolian buffalo mastitic milk in Turkey. We consider that this study may be a helpful tool for future research into mastitis caused by Candida species. Key words: biofilm activity, buffalo, Candida, mastitis Identificirana su bila 66 izolata roda Candida izdvojena iz uzoraka mlijeka upaljenih cetvrti vimena anatolijskih bivolica upotrebom sustava API 20 C AUX. Najcesce izdvojene vrste bile su Candida krusei (27,3%), zatim Candida rugosa (16,7%), Candida kefyr (12,1%) i Candida tropicalis (10,6%). Ostale izdvojene vrste bile su Candida albicans (9,1%), Candida zeylanoides (6,1%), Candida parapsilosis (6,1%), Candida guilliermondi (4,5%), Candida famatta (3,0%), Candida glabratta (3,0%) i Candida ciferrii (1,5%). Ukupno su 53 (80,3%) izolata tvorila biofilm. Svi izolati vrsta C. krusei, C. tropicalis, C. parapsilosis i C. guilliermondii tvorili su biofilm. C. ciferrii nije tvorila biofilm. To je prvo istrazivanje tvorbe biofilma in vitro razlicitih vrsta Candida izdvojenih iz sekreta upaljenog vimena anatolijskih bivolica u Turskoj. Ovo istrazivanje moze biti od koristi u buducim istrazivanjima mastitisa uzrokovanoga vrstama roda Candida. Kljucne rijeci: aktivnost biofilma, bivol, Candida, mastitis