Trypanosoma evansi possesses a gene similar to putative Trypanosoma brucei rhodesiense oligosaccharyl transferase clone I/Trypanosoma evansi ima gen slican genu za oligosaharil-transferazu klona I protozoona Trypanosoma brucei rhodesiense

Recent data has shown that there are strong indications that the putative oligosaccharyl transferase genes from Trypanosoma brucei rhodesiense were conserved within the family Trypanosomatidae. Based on these findings, the study endeavored to determine if Trypanosoma evansi also possessed putative oligosaccharyl transferase (OST) clone I previously documented in Trypanosoma brucei rhodesiense. Using the DNA hybridization method (Southern blot analyses), genomic DNAs of Trypanosoma brucei rhodesiense and Trypanosoma evansi were processed using the same set of restriction enzymes and subsequently hybridized by the same set of DNA probes designed from the reported nucleotide sequence of Trypanosoma brucei rhodesiense putative oligosaccharyl transferase clone I. The results exhibited that Trypanosoma evansi also contains a gene similar to the reported Trypanosoma brucei rhodesiense putative OST gene clone I, as shown by the successful hybridization of the DNA probes to their complementary nucleotide sequences in the genome of the Trypanosoma evansi species. In addition, the data also showed that Trypanosoma brucei rhodesiense and Trypanosoma evansi genomes shared some common restriction sites and loci within the genome of each individual parasite species. Key words: Trypanosoma evansi, oligosaccharyl transferase, N-glycosylation, DNA hybridization Nedavna istrazivanja pokazala su da su geni za oligosaharil-transferazu protozoona brucei rhodesiense vrlo dobro sacuvani unutar porodice Trypanosomatidae rypanosomatidae. Cilj istrazivanja bio je otkriti je li ista pojava karakteristicna za gen za oligosaharil-transferazu klona I protozoona Trypanosoma evansi. Genomske DNA vrste Trypanosoma brucei i vrste Trypanosoma evansi bile su pretrazene hibridizacijom DNA (Southern Blot analizom) rabeci istu skupinu restrikcijskih enzima kao i iste probe za hibridizaciju DNA pripravljene na temelju objavljenog slijeda nukleotida za oligosaharil-transferazu klona I protozoona Trypanosoma brucei rhodesiense. Rezultati su pokazali da Trypanosoma evansi takoder sadrzi gen koji je vrlo sliican genu za oligosaharil-transferazu protozoona Trypanosoma brucei rhodesiense, sto je dokazano uspjesnom hibridizacijom DNA proba s komplementarnim nukleotidnim slijedovima u genomu vrste Trypanosoma evansi. Istrazivanje je pokazalo da Trypanosoma brucei rhodesiense i Trypanosoma evansi dijele i zajednicka estrikcijska mjesta. Kljucne rijeci: Trypanosoma evansi rypanosoma evansi, oligosaharil-tr