Automatic selection of two-dimensional electrophoresis gel with least geometric distortions/Automatine dvimates elektroforezes geliu su mazausiais geometriniais iskraipymais atranka

The paper presents an algorithm for automated selection of the highest quality two-dimensional gel electrophoresis image. The quality criterion is the amount of vertical geometric distortions of the gel. The aim is to select the least distorted gel from the group received during the same biochemical experiment. Vertical geometric distortions displace proteins of the same molecular mass from the horizontal line and have a greater impact on the determination of protein characteristics than horizontal distortions. After presenting algorithm for evaluation of distortions and selection of base gel results are compared to expert's made selections. If necessary, algorithm may be adapted for horizontal distortion evaluation. Keywords: two-dimensional electrophoresis, 2DE, geometric distortions, automatic gel analysis. Straipsnyje pristatomas algoritmas, skirtas automatiniam kokybiskiausio dvimates elektroforezes gelio nustatymui. Kokybes kriterijumi yra vertikaliu geometriniu iskraipymu gelyje kiekis. Is biocheminio eksperimento metu gautos grupes geliu atrenkamas vienas gelis su maziausiais vertikaliais geometriniais iskraipymais. Vertikalus iskraipymai, del kuriu vienodos molekulines mases baltymai issidesto ne horizontaleje, turi didesne itaka baltymu parametru nustatymui. Darbe pristatomas geometriniu iskraipymu ivertinimo algoritmas, jo taikymas atrenkant kokybiskiausia geli bei algoritmo rezultatu palyginimas su eksperto geliu atrankos rezultatais. Reikalui esant algoritmas gali buti adaptuojamas horizontaliu iskraipymu ivertinimui. Reiksminiai zodziai: dvimate elektroforeze, 2ME, geometriniai iskraipymai, automatine geliu analize.