The Effect of Smoking Cessation and Subsequent Resumption on Absorption of Inhaled Insulin

The Effect of Smoking Cessation and Subsequent Resumption on Absorption of Inhaled Insulin Reinhard H.A. Becker , MD, PHD 1 , Sue Sha , MD, PHD 2 , Annke D. Frick , PHD 1 and Robert J. Fountaine , PHARMD 2 1 Aventis Pharma Deutschland, Frankfurt, Germany 2 Pfizer Global Research and Development, Groton, Connecticut Address correspondence and reprint requests to Reinhard H.A. Becker, Clinical Discovery and Human Pharmacology, Aventis Pharma Deutschland, Industriepark Höchst, Building H831, Room C0441, 65926 Frankfurt am Main, Germany. E-mail: reinhard.becker{at}sanofi-aventis.com Abstract OBJECTIVE —To assess the absorption profile of inhaled insulin in healthy, actively smoking subjects at baseline, after smoking cessation, and after smoking resumption and compare it with nonsmoking subjects. RESEARCH DESIGN AND METHODS —Insulin pharmacokinetics and glucodynamics were measured in 20 male smoking subjects (10–20 cigarettes/day) and 10 matched nonsmoking subjects after receiving inhaled insulin (1 mg) or the approximate subcutaneous insulin equivalent (3 units) in a randomized cross-over fashion. All smokers then received inhaled insulin 12 h, 3 days, and 7 days into a smoking cessation period. They then resumed smoking for 2–3 days before again receiving inhaled insulin 1 h after the last cigarette. RESULTS —Before smoking cessation, maximum insulin concentration ( C max ) and area under the curve (AUC) for insulin concentration time (AUC-Insulin 0–360 ) with inhaled insulin were higher, and time to C max ( t max ) shorter, in smokers than nonsmokers ( C max 26.8 vs. 9.7 μU/ml; AUC-Insulin 0–360 2,583 vs. 1,645 μU · ml −1 · min −1 ; t max 20 vs. 53 min, respectively; all P < 0.05), whereas with subcutaneous insulin, systemic exposure was unchanged (AUC-Insulin 0–360 2,324 vs. 2,269 μU · ml −1 · min −1 ; P = NS). After smoking cessation, AUC-Insulin 0–360 decreased with inhaled insulin by up to 50% within 1 week and approached nonsmoker levels. C max decreased and t max increased relative to baseline but were still not comparable with nonsmoker values. Smoking resumption completely reversed the effect of smoking cessation. Glucodynamics corroborated the observed findings in insulin pharmacokinetics. CONCLUSIONS —Cessation and resumption of smoking greatly altered the pharmacokinetics of inhaled insulin. As rapid changes in systemic insulin exposure increase hypoglycemia risk, inhaled insulin should not be used in people with diabetes who choose to continue s