Transcription and Translation Inhibitors Permit Metamorphosis up to Radiole Formation in the Serpulid Polychaete Hydroides elegans Haswell

Settlement and metamorphosis in most well-studied marine invertebrates are rapid processes, triggered by external cues. How this initial environmentally mediated response is transduced into morphogenetic events that culminate in the formation of a functional juvenile is still not well understood for any marine invertebrate. The response of larvae of the serpulid polychaete Hydroides elegans to inhibitors of mRNA and protein synthesis was examined to determine if metamorphosis requires these molecular processes. Competent larvae of H. elegans were induced to metamorphose by exposing them to a bacterial film or a 3-h pulse of 10 mM CsCl in the presence of the gene-transcription inhibitor DRB (5,6-dichloro-1-β-D-ribofuranosylbenzimidazole) or the translation inhibitor emetine. When induced to metamorphose in the presence of either inhibitor, larvae of H. elegans progressed through metamorphosis to the point at which branchial radioles start to develop. DRB and emetine inhibited the incorporation of radiolabeled uridine into RNA and radiolabeled methionine into peptides, respectively, indicating that they were effective in blocking the appropriate syntheses. Taken together, these results indicate that the induction of metamorphosis in H. elegans does not require de novo transcription or translation, and that the form of the juvenile worm is achieved in two phases. During the first phase, larvae respond to the inducer by attaching to the substratum, secreting a primary tube, resorbing the prototroch cilia, undergoing caudal elongation, and differentiating the collar; once the collar is formed, they begin secreting the secondary, calcified tube. During the second phase, the small worm develops branchial radioles and begins to grow, requiring new mRNA and protein syntheses.