A Multiplex Nanopore Sequencing Approach for the Detection of Multiple Arboviral Species

The emergence and continued geographic expansion of arboviruses and the growing number of infected people have highlighted the need to develop and improve multiplex methods for rapid and specific detection of pathogens. Sequencing technologies are promising tools that can help in the laboratory diag...

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Veröffentlicht in:	Viruses 2023, Vol.16 (1)
Hauptverfasser:	Xavier, Joilson, Fonseca, Vagner, Adelino, Talita, Iani, Felipe C. M, Pereira, Glauco C, Duarte, Myrian M, Lima, Mauricio, Castro, Emerson, Oliveira, Carla, Fritsch, Hegger, Guimarães, Natalia, Lamounier, Ludmila O, Barreto, Fernanda Khouri, Braga de Oliveira, Camilo M. M, Maymone Gonçalves, Crhistinne C, Malta Lima, Danielle, de Oliveira, Elaine C, de Castro Lichs, Gislene G, Gomes, Iago, Mazaro, Janaina, Rodrigues, Janete T. N, Abrantes, Jayra, Colares, Jeová K. B, Luz, Kleber G, Barbosa da Silva, Luana, Demarchi, Luiz, Câmara, Magaly C. B, Umaki Zardin, Marina C. S, Sabatini Mello Pinheiro, Rafaela, Barbosa Souza, Rutilene, Haddad, Simone K, Figueiredo da Silva, Stephanni, Slavov, Svetoslav N, Rocha, Themis, Morel, Noelia, Chiparelli, Hector, Burgueño, Analía, Bórmida, Victoria, Co, Martín, Rosario S, Pereira, Allan C, dos Santos, Marcelo F, André Júnior, Walter, Mendez Rico, Jairo, Franco, Leticia, Rosewell, Alexander, do Carmo Said, Rodrigo F, de Albuquerque, Carlos F. C, Noia Maciel, Ethel L, Santini de Oliveira, Marília, Venâncio da Cunha, Rivaldo, Vinhal Frutuoso, Livia C, de Filippis, Ana M. B, Giovanetti, Marta, Carlos Junior Alcantara, Luiz
Format:	Report
Sprache:	eng
Schlagworte:	Arbovirus diseases Arboviruses Causes of DNA sequencing Genetic aspects Identification and classification Methods Nucleotide sequencing Polymerase chain reaction
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Beschreibung
Zusammenfassung:	The emergence and continued geographic expansion of arboviruses and the growing number of infected people have highlighted the need to develop and improve multiplex methods for rapid and specific detection of pathogens. Sequencing technologies are promising tools that can help in the laboratory diagnosis of conditions that share common symptoms, such as pathologies caused by emerging arboviruses. In this study, we integrated nanopore sequencing and the advantages of reverse transcription polymerase chain reaction (RT-PCR) to develop a multiplex RT-PCR protocol for the detection of Chikungunya virus (CHIKV) and several orthoflaviviruses (such as dengue (Orthoflavivirus dengue), Zika (Orthoflavivirus zikaense), yellow fever (Orthoflavivirus flavi), and West Nile (Orthoflavivirus nilense) viruses) in a single reaction, which provides data for sequence-based differentiation of arbovirus lineages.
ISSN:	1999-4915 1999-4915
DOI:	10.3390/v16010023