Antibiofilm Effect of ISiegesbeckia pubescens/I against IS. mutans/I According to Environmental Factors

Siegesbeckia pubescens has long been used as a traditional medicine in Asia. In traditional prescriptions, it has been used mainly for its anti-inflammatory properties. Still, comparative research on its activity according to its origin has not yet been conducted. This study used estimated 30% ethanol extracts of S. pubescens from Yeongcheon, Chungju, and Suncheon in Korea for their antibacterial properties, glucosyltransferase (GTase) activity, organic acid production, biofilm formation, and antioxidant properties. As a result, it was determined that the minimum inhibitory concentration (MIC) against periodontitis-associated pathogens was ≥0.2 mg/mL, the antibiofilm effect was ≥80%, and the minimum IC[sub.50] values of the extracts were 39.64 μg/mL for DPPH and 172.1 μg/mL for the ABTS radical, and 17.73 μg/mL for SOD-like activity. The comparative analysis of climate data confirmed that the activities were inversely proportional to precipitation and humidity and directly proportional to temperature. Therefore, in light of the effect of the environment on the production of more effective and superior varieties, it is anticipated that the value of S. pubescens as a preventive treatment for periodontitis will increase and contribute to the development of more effective varieties.