Glyphosate and aminomethylphosphonic acid levels in water and soil samples from Transylvanian Roma community analyzed by HPLC-FLD method

This paper purpose was to develop a sensitive and selective method for the determination of glyphosate, and aminomethylphosphonic acid (AMPA) residues in water and glyphosate from soil samples. The method involves a derivatization step with 9-fluorenylmethylchloroformate in borate buffer of these compounds and liquid chromatography separation with fluorescence detection (HPLC-FLD). Separation of derivatized glyphosate and AMPA compounds was performed on an Agilent ZORBAX C18 reversedphase column. The mobile phase consisted of a mixture of acetonitrile and 0.05 M K[H.sub.2]P[O.sub.4] solution [30:70 v/v]. Limits of detection (LOD) was 0.28 [micro]g [L.sup.-1] for glyphosate, and 0.35 [micro]g [L.sup.-1] for AMPA, and limits of quantification (LOQ) was 0.84 [micro]g [L.sup.-1] for glyphosate and 1.05 [micro]g [L.sup.-1] for AMPA. The method has been validated for surface water and soil by recovery studies with samples spiked at 25 and 5 [micro]g [L.sup.-1]. In water samples, the mean recoveries values ranged between 86.44 - 103.9% for glyphosate, and 71.27 - 99.08% for AMPA. The mean recoveries values for glyphosate ranged from 57 81.5% in soil samples. The developed method has been applied for determination of these compounds in water and soil samples from agricultural and rural areas of Roma community from some Transylvania Counties. Keywords: Glyphosate, aminomethylphosphonic acid, water, soil, FMOC-CI, RP-HPLC-FLD.