Electrochemical competitive immunodetection of messenger RNA modified with N6-methyladenosine by using DNA-modified mesoporous PtCo nanospheres

N 6 -Methyladenosine (m 6 A) is the most abundant RNA modification in eukaryotic messenger RNA (mRNA). A highly sensitive electrochemical immunosensor is described for the determination of m 6 A-RNA. The method is based on the use of antibody (anti-m 6 A) and PtCo mesoporous nanospheres (MPNs). The...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Mikrochimica acta (1966) 2020-01, Vol.187 (1), p.31, Article 31
Hauptverfasser: Ou, Xinying, Pu, Qinli, Sheng, Shangchun, Dai, Tao, Gou, Dan, Yu, Wen, Yang, Tingyan, Dai, Ling, Yang, Yujun, Xie, Guoming
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:N 6 -Methyladenosine (m 6 A) is the most abundant RNA modification in eukaryotic messenger RNA (mRNA). A highly sensitive electrochemical immunosensor is described for the determination of m 6 A-RNA. The method is based on the use of antibody (anti-m 6 A) and PtCo mesoporous nanospheres (MPNs). The analogously modified probe of type m 6 A-DNA-PtCo competes with m 6 A-RNA for antibodies on the gold electrode as an electrical signal probe. The electrical signal, best acquired at a working potential of −0.37 V (vs. Ag/AgCl) reflects the concentration of m 6 A. The PtCo MPNs catalyze the reduction of H 2 O 2 , and this amplifies the current and enhances sensitivity. The detection time of the assay is
ISSN:0026-3672
1436-5073
DOI:10.1007/s00604-019-4010-8