Electrochemical competitive immunodetection of messenger RNA modified with N6-methyladenosine by using DNA-modified mesoporous PtCo nanospheres
N 6 -Methyladenosine (m 6 A) is the most abundant RNA modification in eukaryotic messenger RNA (mRNA). A highly sensitive electrochemical immunosensor is described for the determination of m 6 A-RNA. The method is based on the use of antibody (anti-m 6 A) and PtCo mesoporous nanospheres (MPNs). The...
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Veröffentlicht in: | Mikrochimica acta (1966) 2020-01, Vol.187 (1), p.31, Article 31 |
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Hauptverfasser: | , , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | N
6
-Methyladenosine (m
6
A) is the most abundant RNA modification in eukaryotic messenger RNA (mRNA). A highly sensitive electrochemical immunosensor is described for the determination of m
6
A-RNA. The method is based on the use of antibody (anti-m
6
A) and PtCo mesoporous nanospheres (MPNs). The analogously modified probe of type m
6
A-DNA-PtCo competes with m
6
A-RNA for antibodies on the gold electrode as an electrical signal probe. The electrical signal, best acquired at a working potential of −0.37 V (vs. Ag/AgCl) reflects the concentration of m
6
A. The PtCo MPNs catalyze the reduction of H
2
O
2
, and this amplifies the current and enhances sensitivity. The detection time of the assay is |
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ISSN: | 0026-3672 1436-5073 |
DOI: | 10.1007/s00604-019-4010-8 |