Development of mutant RsF3'H allele-based marker for selection of purple and red root in radish

To develop a gene-based DNA selection marker for the purple and red colored root characteristics, multiplex-PCR was performed for genotyping of Raphanus sativus flavonoid 3' hydroxylase (RsF3'H) in several cultivars and progenies. In cultivars, DNA with an approximately 450-bp in size was amplified from several radishes, such as purple-, white-, green-, and black-root radishes, whereas approximately 750-bp in size was amplified from red-root radishes. In progenies from red- and white-roots, almost F.sub.1 progenies had purple-root with DNA fragments of approximately 450- and 750-bp. In F.sub.2 progenies, two band patterns which are 450-bp, and both 450- and 750-bp fragments were amplified from purple-root individuals, whereas only 750-bp fragment were amplified from red-root individuals. In contrast, three band patterns were amplified from white-root individuals. Therefore, our data indicated that mutant RsF3'H allele-based marker could select the potential red root genotype effectively in breeding program of purple and red root radishes.